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  3. Ultrasensitive detection of micrococcal nuclease activity and Staphylococcus aureus contamination using optical biosensor technology-A review

Ultrasensitive detection of micrococcal nuclease activity and Staphylococcus aureus contamination using optical biosensor technology-A review

  • Talanta. 2021 May 1:226:122168. doi: 10.1016/j.talanta.2021.122168.
Somayeh Sahraneshin Samani 1,  Amir Khojastehnezhad 2,  Mohammad Ramezani 3,  Mona Alibolandi 3,  Farideh Tabatabaei Yazdi 1,  Sayed Ali Mortazavi 1,  Zahra Khoshbin 2,  Khalil Abnous 4,  Seyed Mohammad Taghdisi 5
Affiliations

Affiliations

  • 1 Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad (FUM), Mashhad, Iran.
  • 2 Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
  • 3 Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
  • 4 Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: taghdisihm@mums.ac.ir.
  • 5 Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: abnouskh@mums.ac.ir.
PMID: 33676710 DOI: 10.1016/j.talanta.2021.122168
Abstract

One of the most common and important pathogenic bacteria is Staphylococcus aureus (S. aureus) which is known as a foodborne illness all over the world. The detection of micrococcal nuclease (MNase) can act as a unique diagnostic biomarker for the identification of S. aureus. So far, various complex methods have been introduced for the evaluation of S. aureus bacterium. However, they have different limitations such as labor-intensive, inaccurate results and time-consuming procedures. Thus, it is of particular attention to develop fast, easy, simple and more approachable detection methods based on nanotechnology and MNase detection. In this review, recent advances and modern techniques of ultrasensitive biosensors based on quantum dots (QDs), noble metal and magnetic nanoparticles (NPs), graphene oxide (GO) nanosheets, and also transfer energy strategy have been discussed for the identification of MNase activity and S. aureus contamination. Besides, advantages and disadvantages of different types of fluorescent, phosphorescent and colorimetric biosensors have been discussed.

Keywords

Colorimetric methods; Fluorescent biosensors; Micrococcal nuclease activity; Staphylococcus aureus.

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