2. Academic Validation
  3. Ultrasensitive and green electrochemical immunosensor for mycotoxin ochratoxin A based on phage displayed mimotope peptide

Ultrasensitive and green electrochemical immunosensor for mycotoxin ochratoxin A based on phage displayed mimotope peptide

  • Talanta. 2019 Mar 1:194:919-924. doi: 10.1016/j.talanta.2018.10.081.
Si-Lu Hou 1,  Zhen-E Ma 1,  Hui Meng 1,  Yang Xu 1,  Qing-Hua He 2
Affiliations

Affiliations

  • 1 State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China.
  • 2 State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China. Electronic address: heqinghua@ncu.edu.cn.
PMID: 30609625 DOI: 10.1016/j.talanta.2018.10.081
Abstract

Here, we demonstrated a new approach for development of an ultrasensitive and green electrochemical immunosensor for Ochratoxin A (OTA). Phage displayed mimotope peptide of OTA was used as mimics of conventional competing antigen, which is chemical synthesized with toxic mycotoxins OTA as raw material, in a competitive sensing platform. The working electrode was modified by polyethylene glycol (PEG) for the purpose of immobilizing antibody effectively. Under the optimized test condition, the limit of detection (LOD) of the established immunosensor was 2.04 fg/mL, and the linear range was 7.17-548.76 fg/mL. Specific measurement of this established method was conducted by testing cross-reactivity of Other common mycotoxins, the result showed that mimotope peptide-based immunosensor has negligible cross-reactivity with Other mycotoxins. Furthermore, the novel concept of phage displayed mimotope peptide-based immunosensor may provide a potential application in general method for the ultrasensitive detection of various toxic small molecules in food.

Keywords

Electrochemical immunosensor; Ochratoxin A; Phage displayed peptide.

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