2. Academic Validation
  3. Analytical strategy for determination of known and unknown destruxins using hybrid quadrupole-Orbitrap high-resolution mass spectrometry

Analytical strategy for determination of known and unknown destruxins using hybrid quadrupole-Orbitrap high-resolution mass spectrometry

  • Anal Bioanal Chem. 2017 May;409(13):3347-3357. doi: 10.1007/s00216-017-0276-z.
Natalia Arroyo-Manzanares 1 2,  José Diana Di Mavungu 3,  Inmaculada Garrido-Jurado 4,  Lourdes Arce 5,  Lynn Vanhaecke 6,  Enrique Quesada-Moraga 4,  Sarah De Saeger 7
Affiliations

Affiliations

  • 1 Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ghent University, Ottergemsesteenweg 460, 9000, Ghent, Belgium. qa2arman@uco.es.
  • 2 Department of Analytical Chemistry, University of Cordoba, Annex C3 Building, Campus of Rabanales, 14071, Cordoba, Spain. qa2arman@uco.es.
  • 3 Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ghent University, Ottergemsesteenweg 460, 9000, Ghent, Belgium. Jose.DianaDiMavungu@UGhent.be.
  • 4 Department of Agricultural and Forestry Sciences, ETSIAM, University of Cordoba, Building C4, Campus of Rabanales, 14071, Cordoba, Spain.
  • 5 Department of Analytical Chemistry, University of Cordoba, Annex C3 Building, Campus of Rabanales, 14071, Cordoba, Spain.
  • 6 Faculty of Veterinary Medicine, Department of Veterinary Public Health and Food Safety, Laboratory of Chemical Analysis, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium.
  • 7 Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ghent University, Ottergemsesteenweg 460, 9000, Ghent, Belgium.
PMID: 28280843 DOI: 10.1007/s00216-017-0276-z
Abstract

An analytical strategy based on a hybrid quadrupole-Orbitrap mass spectrometry was proposed for the simultaneous screening of known destruxins and characterization of potential members of this class of secondary metabolites, in order to evaluate the metabolite production of entomopathogenic fungi used as biocontrol agents. Initially, the fragmentation pathway of the known and commercially available destruxin A was established combining high resolution mass spectrometry (HRMS) and multiple stage MS data in order to obtain the strategy for the characterization of Other destruxins for which reference standards were not available. Nineteen known destruxins including A, B, C, D, Ed, F, A1, B1, Ed1, A2, B2, D2, A3, DesmA, DesmB, DesmC, DesmB2, and two chloro-derivatives (Cl and E2 chlorohydrin) were unequivocally identified in Metarhizium brunneum using the proposed strategy. In addition, four unknown destruxins, namely C1, Ed2, G, and G1, were structurally elucidated and characterized for the first time in this Fungal strain.

Keywords

Destruxins identification; Fragmentation pathway; Hybrid quadrupole-Orbitrap; Untargeted analysis.

Figures
Products