2. Academic Validation
  3. Osteoblast-Targeting-Peptide Modified Nanoparticle for siRNA/microRNA Delivery

Osteoblast-Targeting-Peptide Modified Nanoparticle for siRNA/microRNA Delivery

  • ACS Nano. 2016 Jun 28;10(6):5759-68. doi: 10.1021/acsnano.5b07828.
Yao Sun 1 2 3,  Xiongzhen Ye 4,  Mingxiang Cai 1,  Xiangning Liu 5,  Jia Xiao 4,  Chenyang Zhang 1,  Yayu Wang 4,  Li Yang 4,  Jiafan Liu 4,  Shannai Li 4,  Chen Kang 3,  Bin Zhang 3,  Qi Zhang 1,  Zuolin Wang 1 2,  An Hong 4,  Xiaogang Wang 4
Affiliations

Affiliations

  • 1 Department of Oral Implantology, School of Stomatology, Tongji University , Shanghai 200072, China.
  • 2 Shanghai Engineering Research Center of Tooth Restoration and Regeneration , Shanghai 200072, China.
  • 3 Sino-Russian Institute of Hard Tissue Development and Regeneration, The Second Affiliated Hospital of Harbin Medical University , Harbin 150086, China.
  • 4 Department of Cell Biology & Institute of Biomedicine, College of Life Science and Technology, Jinan University , Guangzhou 510632, China.
  • 5 The First Affiliated Hospital of Jinan University , Guangzhou 510632, China.
PMID: 27176123 DOI: 10.1021/acsnano.5b07828
Abstract

Antiosteoporosis gene-based drug development strategies are presently focused on targeting osteoblasts to either suppress bone loss or increase bone mass. Although siRNA/microRNA-based gene therapy has enormous potential, it is severely limited by the lack of specific cell-targeting delivery systems. We report an osteoblast-targeting peptide (SDSSD) that selectively binds to osteoblasts via periostin. We developed SDSSD-modified polyurethane (PU) nanomicelles encapsulating siRNA/MicroRNA that delivers drugs to osteoblasts; the data showed that SDSSD-PU could selectively target not only bone-formation surfaces but also osteoblasts without overt toxicity or eliciting an immune response in vivo. We used the SDSSD-PU delivery system to deliver anti-miR-214 to osteoblasts and our results showed increased bone formation, improved bone microarchitecture, and increased bone mass in an ovariectomized osteoporosis mouse model. SDSSD-PU may be a useful osteoblast-targeting small nucleic acid delivery system that could be used as an anabolic strategy to treat osteoblast-induced bone diseases.

Keywords

bone formation; gene delivery; osteoblast targeting peptide; osteoporosis therapy; polyurethane nanoparticle.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-P11070
    Osteoblast-Targeting-Peptide