2. Academic Validation
  3. Solanesol protects human hepatic L02 cells from ethanol-induced oxidative injury via upregulation of HO-1 and Hsp70

Solanesol protects human hepatic L02 cells from ethanol-induced oxidative injury via upregulation of HO-1 and Hsp70

  • Toxicol In Vitro. 2015 Apr;29(3):600-8. doi: 10.1016/j.tiv.2015.01.009.
Xiangyang Yao 1,  Qin Bai 2,  Dazhong Yan 3,  Guilan Li 4,  Chaotian Lü 2,  Hui Xu 2
Affiliations

Affiliations

  • 1 Department of Biology and Food Engineering, Bengbu College, Bengbu, PR China. Electronic address: leeyxy@gmail.com.
  • 2 Department of Biology and Food Engineering, Bengbu College, Bengbu, PR China.
  • 3 School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, PR China.
  • 4 College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, PR China.
PMID: 25645596 DOI: 10.1016/j.tiv.2015.01.009
Abstract

In present study, we showed that the mRNA and protein levels of HO-1 and HSP70 in solanesol-treated L02 cells were significantly increased. The induction of the HO-1 by solanesol is majorly achieved via enhancing the nuclear translocation and transactivity of Nrf2 through enhancement of Hsp90-Keap1 interaction, while solanesol-elevated HSP70 is related with promoting the nuclear translocation of HSF1 through the involvement of chaperones interaction. Furthermore, the induction of HO-1 and HSP70 by solanesol could protect against ethanol-induced liver injury, including significantly suppressing the elevation of the activities of LDH and AST, attenuating ethanol-induced increase of the MDA, ROS level and decrease of the GSH level. Moreover, solanesol also suppressed ethanol-induced Apoptosis of L02 cells by inhibition of nuclear morphological damage, procaspase 3 and cleavage of Caspase 3 and PARP, suggesting solanesol may be beneficial against ALD. Solanesol also promoted tBHQ-mediated protective effects. However, treatment cells with SnPP or PES markedly abrogated the protective effects of solanesol on ethanol-induced cell injury. These results strongly suggested that solanesol could protect ethanol-induced L02 cell damage, which might be attributed to the activation of HO-1 and HSP70.

Keywords

HO-1; HSF1; Hsp70; Nrf2; Solanesol.

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