2. Academic Validation
  3. [Quantitative analysis of midecamycin by HPLC]

[Quantitative analysis of midecamycin by HPLC]

  • Yao Xue Xue Bao. 1991;26(2):156-9.
H He 1,  R G Yu,  Q H Yang,  Z D Yu,  X H Wang
Affiliations

Affiliation

  • 1 China Pharmaceutical University, Nanjing.
PMID: 1950573
Abstract

A reversed phase high performance liquid chromatographic method for the assay of midecamycin was developed. The method used a Hitachi Gel 3050 column at 50 degrees C and a mobile phase of methanol--0.01 mol/L phosphate buffer solution at pH 5.8 (45:55). The column effluent was monitored Z at 231 nm. SF-837A1, leucomycin A6 and minor components could be separated in less than 15 min. The retention times of SF-837A1 and leucomycin A6 are 7 and 10 min respectively. The method is very simple and rapid.

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