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  4. Basal Cell Culture Media
  5. DMEMF-12 (1:1), L-Glutamine, Phenol Red, HEPES (Powder)

DMEMF-12 (1:1), L-Glutamine, Phenol Red, HEPES (Powder) 

Cat. No.: HY-K3002C
Manual COA SDS Technical Support

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cell successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, etc.

DMEMF-12 (1:1), L-Glutamine, Phenol Red, HEPES (Powder)
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  • Description

  • Storage

  • Attention

  • Documentation

Description
& Advantages

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cell successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, etc. This product is a 1:1 mixture of DMEM and Ham’s F-12, combining DMEM’s high concentration of amino acids and vitamins with F-12’s wide variety of components. This product is suitable for cell culture under low serum conditions.

This product is provided in a powder form, offering excellent stability and ease of transport, making it suitable for large-scale preparation and long-term storage.

MCE offers a range of different formulations of DMEM medium for different cell culture applications.

Storage

4°C, 3 years.

Dry and protect from light.

Attention

1. To maintain optimal product performance, please store under the recommended conditions.

2. Sodium bicarbonate is prone to decomposition; therefore, it is provided as a separate component and should be added during medium preparation at a final concentration of 1.2 g/L.

3. It is recommended to prepare 10 L of medium at once. Partial preparation may cause the remaining powder to absorb moisture, potentially affecting the quality of the medium.

4. This product is for R&D use only, not for drug, household, or other uses.

5. For your safety and health, please wear a lab coat and disposable gloves to operate.

Documentation

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
DMEMF-12 (1:1), L-Glutamine, Phenol Red, HEPES (Powder)
Cat. No.:
HY-K3002C
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