2. Apoptosis
  3. Ferroptosis
  4. Ferroptosis-IN-8

Ferroptosis-IN-8 is a potent ferroptosis inhibitor with an EC50 of 40.49 nM. Ferroptosis-IN-8 effectively reduces lipid ROS levels in cells. Ferroptosis-IN-8 act as an antioxidant by capturing lipid radicals, leading to a reduction in the accumulation of harmful lipid peroxides and, ultimately, inhibiting ferroptosis.

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Ferroptosis-IN-8

Ferroptosis-IN-8 Chemical Structure

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Ferroptosis-IN-8 Related Antibodies

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Description

Ferroptosis-IN-8 is a potent ferroptosis inhibitor with an EC50 of 40.49 nM. Ferroptosis-IN-8 effectively reduces lipid ROS levels in cells. Ferroptosis-IN-8 act as an antioxidant by capturing lipid radicals, leading to a reduction in the accumulation of harmful lipid peroxides and, ultimately, inhibiting ferroptosis[1].

IC50 & Target

IC50: 40.49 nM (Ferroptosis)
In Vitro

Ferroptosis-IN-8 (Compound B4) (200 nM) effectively rescues HKC, HK-2, Khos, SW480, and HTR-8/SVneo cells from ferroptosis induced by RSL3 or FIN56[1].
Ferroptosis-IN-8 (Compound B4) (200 nM) againstes ferroptosis predominantly relies on their intrinsic antioxidative capacity, rather than classical signaling pathways including lipid oxidation metabolism[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ferroptosis-IN-8 Related Antibodies

Cell Viability Assay[1]

Cell Line: HKC, HK-2, HT-1080, Khos, SW480, and HTR-8/SVneo cells treatment with RSL3 or FIN56
Concentration: 200 nM
Incubation Time: 24 h
Result: Effectively rescued HKC, HK-2, HT-1080, Khos, SW480, and HTR-8/SVneo cells from ferroptosis induced by RSL3 or FIN56.

Real Time qPCR[1]

Cell Line: HT-1080 cell
Concentration: 200 nM
Incubation Time:
Result: Didn't significantly affect the protein levels of key factors related to lipid metabolism (ACSL4, ALOX15, and COX2), glutathione metabolism pathways (GPX4, SLC7A11), and iron metabolism (DMT1, TFRC, and FTH1)
In Vivo

Ferroptosis-IN-8 (Compound B4) (10 mg/kg, twice, 1 h before ischemia onset, i.p.) alleviates tissue damage in a mouse model of renal ischemia-reperfusion injury[1].
Ferroptosis-IN-8 (Compound B4) (10 mg/kg, i.p.) alleviates acetaminophen induced acute liver injury in mice by inhibiting ferritin [1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: acetaminophen-induced acute liver injury mice model[1].
Dosage: 10 mg/kg
Administration: intraperitoneal injection (i.p.)
Result: Attenuated the increased plasma ALT and AST levels induced by acetaminophen, effectively reduced the injury response induced by acetaminophen[1].
Animal Model: kidney ischemia−reperfusion injury mice model[1].
Dosage: 10 mg/kg, twice, 1 h before ischemia onset.
Administration: intraperitoneal injection (i.p.)
Result: Reduced the levels of creatinine and BUN and alleviated the increase in KIM-1 levels in the I/R model[1].
Animal Model:
Dosage:
Administration:
Result:
Animal Model:
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Molecular Weight

285.30

Formula

C15H15N3O3
Appearance

Solid

Color

Yellow to orange

SMILES

NC1=C(C=CC=C1O)C(N/N=C/C2=CC=C(C=C2)OC)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (175.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5051 mL 17.5254 mL 35.0508 mL
5 mM 0.7010 mL 3.5051 mL 7.0102 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5051 mL 17.5254 mL 35.0508 mL 87.6271 mL
5 mM 0.7010 mL 3.5051 mL 7.0102 mL 17.5254 mL
10 mM 0.3505 mL 1.7525 mL 3.5051 mL 8.7627 mL
15 mM 0.2337 mL 1.1684 mL 2.3367 mL 5.8418 mL
20 mM 0.1753 mL 0.8763 mL 1.7525 mL 4.3814 mL
25 mM 0.1402 mL 0.7010 mL 1.4020 mL 3.5051 mL
30 mM 0.1168 mL 0.5842 mL 1.1684 mL 2.9209 mL
40 mM 0.0876 mL 0.4381 mL 0.8763 mL 2.1907 mL
50 mM 0.0701 mL 0.3505 mL 0.7010 mL 1.7525 mL
60 mM 0.0584 mL 0.2921 mL 0.5842 mL 1.4605 mL
80 mM 0.0438 mL 0.2191 mL 0.4381 mL 1.0953 mL
100 mM 0.0351 mL 0.1753 mL 0.3505 mL 0.8763 mL
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Ferroptosis-IN-8 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ferroptosis-IN-8FerroptosisFerroptosis inhibitorsRSL3lipid ROS levelskidney (I/R) injuryacute liver injuryInhibitorinhibitorinhibit

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