2. Metabolic Enzyme/Protease
  3. Cytochrome P450
  4. ε-​Viniferin

ε-​Viniferin  (Synonyms: epsilon-Viniferin)

Cat. No.: HY-N3841 Purity: ≥98.0%
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COA
Handling Instructions Technical Support

ε-Viniferin (epsilon-Viniferin), the dimer of Resveratrol and can be isolated from Vitis vinifera, displays a potent inhibitory for all the CYP activities, with Ki values from 0.5-20 μM. ε-Viniferin possesses potent antioxidant, anti-inflammatory, anti-diabetic, and anti-neurodegenerative capacity.

For research use only. We do not sell to patients.

ε-​Viniferin Chemical Structure

ε-​Viniferin Chemical Structure

CAS No. : 62218-08-0

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of ε-​Viniferin:

ε-​Viniferin Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE ε-​Viniferin

View All Cytochrome P450 Isoform Specific Products:

View All Isoforms
CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A4 CYP17A1

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

ε-Viniferin (epsilon-Viniferin), the dimer of Resveratrol and can be isolated from Vitis vinifera, displays a potent inhibitory for all the CYP activities, with Ki values from 0.5-20 μM. ε-Viniferin possesses potent antioxidant, anti-inflammatory, anti-diabetic, and anti-neurodegenerative capacity[1][2][3].

IC50 & Target

CYP2B6

3 μM (Ki, (BROD))

CYP1A2

5 μM (Ki, (EROD))

CYP3A4

10 μM (Ki, (TST))

CYP4A

15 μM (IC50, (LwOH))

CYP2E1

25 μM (IC50, (CHZ))

CYP2A6

60 μM (Ki, (COH))

Cellular Effect
Cell Line Type Value Description References
A549 CC50
54.7 μM
Compound: (+)-Epsilon-Viniferin
Cytotoxicity against human A549 cells assessed as reduction in cell viability by Alamar blue assay
Cytotoxicity against human A549 cells assessed as reduction in cell viability by Alamar blue assay
[PMID: 32652408]
BTI-TN-5B1-4 IC50
19.6 μM
Compound: Epsilon-Viniferin
Inhibition of human recombinant MAO-A expressed in baculovirus infected BTI-TN-5B1-4 insect cells using p-tyramine as substrate after 15 mins by resorufin dye-based spectrometric analysis
Inhibition of human recombinant MAO-A expressed in baculovirus infected BTI-TN-5B1-4 insect cells using p-tyramine as substrate after 15 mins by resorufin dye-based spectrometric analysis
[PMID: 30686752]
In Vitro

ε-​Viniferin (1-15 μM, 24-72 h) inhibits proliferation of HOS, U2OS, and A549 cells[3].
ε-​Viniferin (20 μM, 24 h) induces DNA fragmentation and nuclear condensation in HOS, U2OS, A549 cells[3].
ε-​Viniferin (10 μM, 1 h) inhibits TGF-β1 induced EMT, invasion and migration, and reverses TGF-β1-induced ROS, MMP2, vimentin, Zeb1, Snail, p-SMAD2, p-SMAD3, and ABCG2 expression in A549 cells[4].
ε-​Viniferin (10 μM, 3 min) inhibits fMLP (0.1 μM)-induced superoxide anion production in human neutrophils, as well as fMLP-induced phosphorylation of ERK, Akt, Src or intracellular calcium mobilization[5].
ε-​Viniferin (50 nM-10 μM, 48 h) protects cells against Rotenone (HY-B1756)-induced neurotoxicity in SH-SY5Y cells via the SIRT3/FOXO3 pathway[6].
ε-​Viniferin (1 μM, 24 h) inhibits Rotenone (HY-B1756)-induced mitochondrial depolarization and oxidative stress in SH-SY5Y cells[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ε-​Viniferin Related Antibodies

Western Blot Analysis[4]

Cell Line: A549 cells
Concentration: 10 μM
Incubation Time: 1 h
Result: Inhibited TGF-β1-induced MMP2, vimentin, Zeb1, p-SMAD2, p-SMAD3, and ABCG2 expression.
In Vivo

ε-​Viniferin (5 mg/kg, i.p., 5 days per week for 4 weeks) inhibits tumor growth in A549 cell xenograft nude mice model without hepatic and kidney injury, and inhibits tumor metastasis in A549 cell lung metastasis mouse model[3][4].
ε-​Viniferin (0.2% in diet, 4 weeks) prevents diet-induced obesity in mice[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: A549 cell xenograft nude mice model[3]
Dosage: 5 mg/kg
Administration: i.p., 5 days per week for 4 weeks
Result: Inhibited tumor weight and volume.
Induced apoptosis in tumor sections.
Decreased serum ALT, AST, bilirubin, and creatinine levels.
Molecular Weight

454.47

Formula

C28H22O6
CAS No.
Appearance

Solid

Color

Off-white to gray

SMILES

OC1=CC([C@H]2[C@H](C3=CC=C(O)C=C3)OC4=CC(O)=CC(/C=C/C5=CC=C(O)C=C5)=C24)=CC(O)=C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 50 mg/mL (110.02 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2004 mL 11.0018 mL 22.0037 mL
5 mM 0.4401 mL 2.2004 mL 4.4007 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (2.75 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.25 mg/mL (2.75 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥98.0%

SDS (252 KB)

COA (258 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2004 mL 11.0018 mL 22.0037 mL 55.0091 mL
5 mM 0.4401 mL 2.2004 mL 4.4007 mL 11.0018 mL
10 mM 0.2200 mL 1.1002 mL 2.2004 mL 5.5009 mL
15 mM 0.1467 mL 0.7335 mL 1.4669 mL 3.6673 mL
20 mM 0.1100 mL 0.5501 mL 1.1002 mL 2.7505 mL
25 mM 0.0880 mL 0.4401 mL 0.8801 mL 2.2004 mL
30 mM 0.0733 mL 0.3667 mL 0.7335 mL 1.8336 mL
40 mM 0.0550 mL 0.2750 mL 0.5501 mL 1.3752 mL
50 mM 0.0440 mL 0.2200 mL 0.4401 mL 1.1002 mL
60 mM 0.0367 mL 0.1834 mL 0.3667 mL 0.9168 mL
80 mM 0.0275 mL 0.1375 mL 0.2750 mL 0.6876 mL
100 mM 0.0220 mL 0.1100 mL 0.2200 mL 0.5501 mL
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ε-​Viniferin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ε-​Viniferin62218-08-0epsilon-ViniferinCytochrome P450CYPsantioxidantanti-inflammatoryanti-diabeticanti-neurodegenerativeInhibitorinhibitorinhibit

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