Tau Antibody (YA5461)

Tau Antibody (YA5461) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Tau.

Tau is a protein that promotes microtubule assembly and stability, potentially involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules while the N-terminus interacts with neural plasma membrane components, indicating its role as a linker protein between these structures. Axonal polarity is predetermined by the localization of TAU/MAPT within the centrosome-defined cell body domain in neurons. Short Tau isoforms provide cytoskeletal plasticity, whereas longer isoforms may primarily contribute to its stabilization. Similar functions include microtubule binding and neuronal morphology regulation (by similar).

P10636

Observed band size: 50-85 kDa

affinity chromatography.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Rat, Mouse

WB: 1:1000-2000; IHC: 1:100-200; ICC/IF: 1:50-200

• 
  Immunohistochemical analysis of paraffin-embedded rat brain tissue using Tau Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded rat brain tissue using Tau Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Synthetic Peptide of Tau