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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D1656
    BDP 581/591 carboxylic acid
    		99.65%
    BDP 581/591 carboxylic acid is a fluorescent dye (Ex=585 nm, Em=594 nm). BDP 581/591 carboxylic acid has a free carboxylic acid group, which can be catalyzed by a catalyst (such as EDC or HATU) to react with primary amines to form stable amide bonds. BDP 581/591 carboxylic acid is highly photostable and can be used for ROS detection.
    BDP 581/591 carboxylic acid
  • HY-D2289
    CalFluor 647 azide
    CalFluor 647 azide is a fluorogenic azide probe that is activated by Cu-catalyzed or metal-free click reaction. CalFluor 647 azide is not fluorescent until it is reacted with alkynes.
    CalFluor 647 azide
  • HY-W111907
    3,3-Diethylthiacarbocyanine iodide
    		99.57%
    3,3-Diethylthiacarbocyanine iodide is a cyanine dye. 3,3-Diethylthiacarbocyanine iodide can be used in photochemical methods.
    3,3-Diethylthiacarbocyanine iodide
  • HY-D1491
    Fast Red Violet LB
    Fast Red Violet LB is a dye for staining tartrate resistant acid phosphatase (TRAP). Fast Red Violet LB can be used for alkaline phosphatase (ALP) activity staining.
    Fast Red Violet LB
  • HY-W040209
    2-(Naphthalen-1-yl)-5-phenyloxazole
    		99.73%
    2-(Naphthalen-1-yl)-5-phenyloxazole (α-NPO) is an organic fluorescent dye. 2-(Naphthalen-1-yl)-5-phenyloxazole has a nitrogen atom in its structure that can interfere with the nitrogen content from the amination step.
    2-(Naphthalen-1-yl)-5-phenyloxazole
  • HY-D1882
    Cy5.5 hydrazide
    Cy5.5 hydrazide is a fluorescent dye containing a hydrazide functionality. Cy5.5 hydrazide can be used to label aldehydes and ketones.
    Cy5.5 hydrazide
  • HY-W110905
    Naphthol green B
    Naphthol green B is an iron-complex dye. Naphthol green B can be used in wool dyeing, silk dyeing and polyamide dyeing. Naphthol green B can be used to fabricate electrochemical sensor for the detection of H2O2.
    Naphthol green B
  • HY-D1886
    Vari Fluor 647 SE
    Vari Fluor 647 SE is a fluorescent dye, SE stands for "succinimidyl ester". Vari Fluor 647 SE belongs to the Vari Fluor family of labeling reagents used in cell and molecular biology research. Vari Fluor 647 SE can react with an amino group to form a covalent bond, thereby introducing Vari Fluor 647 dye into the target molecule or cell. Vari Fluor 647 SE is a reactive dye that produces a fluorescent signal after binding to a target molecule or cell.
    Vari Fluor 647 SE
  • HY-D0053A
    6-ROX hydrochloride
    		99.05%
    6-ROX (6-Carboxy-X-rhodamine) hydrochloride, a fluorescent marker of oligonucleotides, acts as a receptor coupled to 5-FAM and as a donor in FRET imaging. Excitation wavelength: 568 nm. Emission wavelength: 568 nm.
    6-ROX hydrochloride
  • HY-D1423
    Dibromobimane
    		98.52%
    Dibromobimane is a thiol-selective fluorescent imaging agent. Dibromobimane is used to crosslink cysteine- and homocysteine-containing peptides.
    Dibromobimane
  • HY-D1604
    BODIPY FL Ethylamine
    		98.06%
    BODIPY FL Ethylamine is a fluorescent dye that reversibly reacts with aldehyde/ketone to yield a Schiff base. BODIPY FL Ethylamine can be reduced to form a stable amine derviative using sodium borohydride or sodium cyanoborohydride.
    BODIPY FL Ethylamine
  • HY-133852
    FD-1080
    FD-1080 is a fluorophore with both excitation and emission in the NIR-II region (Ex=1064 nm, Em=1080 nm). FD-1080 can be used for in vivo imaging.
    FD-1080
  • HY-D0626
    Pigment Yellow 154
    		98.89%
    Pigment Yellow 154 is a fluorescent dye. Pigment Yellow 154 can be used as biological dye.
    Pigment Yellow 154
  • HY-D1305
    ATTO 488 carboxylic acid
    ATTO 488 carboxylic acid is a new fluorescent label based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 488 carboxylic acid is a carboxylic acid derivative of ATTO 488, which can be used to label proteins or antibodies.
    ATTO 488 carboxylic acid
  • HY-NP0173
    Aleuria Aurantia Lectin (Biotinylated)
    Aleuria Aurantia Lectin (AAL) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Aleuria Aurantia Lectin (AAL) Biotinylated is a biological material or organic compound that can be used in life science research.
    Aleuria Aurantia Lectin (Biotinylated)
  • HY-D1669
    Biotin-11-UTP
    Biotin-11-UTP is a Biotin-labeled uridine triphosphate (UTP), containing 11 atoms in the linker between biotin and UTP. Biotin-11-UTP can biotinylate transcribed RNA in vitro and has been widely used as labeling RNA probe. Biotin-11-UTP is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
    Biotin-11-UTP
  • HY-111956
    D-Ala-Lys-AMCA
    D-Ala-Lys-AMCA is a known proton-coupled oligopeptide transporter 1 (PEPT1) substrate that emits blue fluorescence. D-Ala-Lys-AMCA may be transported into liver cancer cells and Caco-2 cells based on fluorescence analysis. D-Ala-Lys-AMCA can be used for characterizing PEPT1-specific substrates or inhibitors (Ex/Em = 390/480 nm).
    D-Ala-Lys-AMCA
  • HY-D0798
    Catechol violet
    Catechol violet is an efficient and versatile ligand for Cu(I)-catalyzed C-S coupling reactions. Catechol violet is also a complexometric indicator dye.
    Catechol violet
  • HY-133535
    PA Janelia Fluor® 646, SE
    		99.0%
    PA Janelia Fluor® 646, SE (PA-JF646-NHS), a photoactivatable fluorescent dye, is an NHS ester for coupling to primary amine groups. PA-JF646-NHS is non-fluorescent until activated at 365 nm. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g.HaloTag® and SNAP-tag®. PA-JF646-NHS is used for single molecule tracking and super resolution microscopy in live cells, specifically live cell sptPALM and fixed cell PALM. (λExcitation/emission~650/664 nm). Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.
    PA Janelia Fluor® 646, SE
  • HY-NP164B
    Concanavalin A-AF488
    Concanavalin A-AF488 (Con A-AF488) is an AF488-labled Concanavalin A (HY-P2149). Concanavalin A is a Ca2+/Mn2+-dependent and mannose/glucose-binding plant lectin. Concanavalin A binds the N- and O-glycosylated proteins of head and neck carcinoma cells glycoproteins.
    Concanavalin A-AF488
Cat. No. Product Name / Synonyms Application Reactivity