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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D1791
    Vari Fluor 750 SE
    Vari Fluor 750 SE (VF 750 SE) is a dye marker of the Vari Fluor SE series (Ex/Em=747 nm/770 nm). The Vari Fluor SE series of dyes are a class of fluorescent dyes containing NHS ester groups used to label free amines (-NHX) on antibodies, proteins, peptides, amine-modified oligonucleotides and other biomolecules.
    Vari Fluor 750 SE
  • HY-D1621
    3-NBD-C12 Cholesterol
    C-12 NBD cholesterol is a cholesterol derivative with a hydrophilic fluorophore tag (NBD: Ex=465 nm, Em=535 nm). C-12 NBD cholesterol can be used to study the utilization and metabolism of cholesterol.
    3-NBD-C12 Cholesterol
  • HY-D0068
    DEAC, SE
    		99.00%
    DEAC, SE (7-Diethylaminocoumarin-3-carboxylic acid, SE) is an excellent blue fluorescent building block for labeling amine-containing biomolecules.
    DEAC, SE
  • HY-W035138
    Tetra(p-bromophenyl)porphyrin
    Tetra(p-bromophenyl)porphyrin (compound 5c) is a fluorescent dye. Tetra(p-bromophenyl)porphyrin can be used for the synthesis of perfuoroalkyl-substituted tetrakisphenylporphyrins.
    Tetra(p-bromophenyl)porphyrin
  • HY-151751
    BDP TMR alkyne
    		98.08%
    BDP TMR alkyne is an alkyne-containing click chemistry reagent that can click chemistry with azides. BDP TMR alkyne has the fluorophore BDP and can be used for oligonucleotide labeling and amino acid sequencing.
    BDP TMR alkyne
  • HY-P10094
    β-Ala-Gly-Arg-pNA
    		99.10%
    β-Ala-Gly-Arg-pNA is a chromogenic substrate of thrombin with pNA a strong absorbance at 405 nm.
    β-Ala-Gly-Arg-pNA
  • HY-D1154
    β-Ala-Lys(AMCA)
    		99.33%
    β-Ala-Lys(AMCA) is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells.
    β-Ala-Lys(AMCA)
  • HY-D1257
    Msr-Ratio
    		99.13%
    Msr-Ratio (Msr-green) is a ratiometric fluorescent probe of methionine sulfoxide reductase (λex=375 nm, λem=550 nm). Msr-Ratio is used for monitoring the enzyme activity in vitro and in live cells.
    Msr-Ratio
  • HY-D1490
    Fast red TR salt hemi(zinc chloride)
    Fast red TR salt hemi (zinc chloride) is a diazonium dye with red color.
    Fast red TR salt hemi(zinc chloride)
  • HY-N0573R
    Umbelliferone (Standard)
    Umbelliferone (Standard) (7-Hydroxycoumarin (Standard)) is the analytical standard of Umbelliferone (HY-N0573). This product is intended for research and analytical applications. Umbelliferone (7-Hydroxycoumarin), a natural orally active product of the coumarin family, is a fluorescing compound which can be used as a sunscreen agent. Umbelliferone induces cell cycle arrest, apoptosis and DNA fragmentation in HepG2 cells. Umbelliferone exhibits significant anticancer effects. Umbelliferone attenuates the alteration characteristics of allergic airway inflammation. Umbelliferone displays the neuroprotective effects and cross the blood-brain barrier. Umbelliferone exhibits anti-inflammatory and antioxidant effects in chronic alcohol-fed rats.
    Umbelliferone (Standard)
  • HY-131030
    Janelia Fluor® 669, SE
    Janelia Fluor 669, SE (JF669, SE), a red fluorescent dye, can be directly reacted with the available thiol-containing HaloTag ligand under mild conditions (DIEA, DMF) to afford a JF669HaloTag ligand in a single step (Ex = 669 nm; Em = 682 nm). Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.
    Janelia Fluor® 669, SE
  • HY-153080
    Firefly Luciferase mRNA(N1-Me-Pseudo UTP)
    Firefly luciferase mRNA will express a luciferase protein, originally isolated from the Photinus pyralis (Firefly). Firefly Luciferase is a frequently used bioluminescent reporter for gene regulation and function study. It is applicable in assays for gene expression, cell viability and in vivo imaging etc.
    Firefly Luciferase mRNA(N1-Me-Pseudo UTP)
  • HY-153082
    Firefly Luciferase circRNA
    Firefly Luciferase circRNA will express a luciferase protein, originally isolated from the Photinus pyralis (Firefly). Firefly Luciferase circRNA is ideal for monitoring and optimizing transfection efficiency and is recommended as a positive control for circular RNA transfections.
    Firefly Luciferase circRNA
  • HY-117070
    TO-PRO-3 iodide
    		99.00%
    TO-PRO-3 iodide is a highly efficient blue fluorescent dye that can stain cell nucleus as a cell tracer.
    TO-PRO-3 iodide
  • HY-NP0159
    Wheat Germ Agglutinin (Rhodamine)
    Wheat Germ Agglutinin (WGA) Rhodamine is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Wheat Germ Agglutinin (WGA) Rhodamine is a biological material or organic compound that can be used in life science research.
    Wheat Germ Agglutinin (Rhodamine)
  • HY-23926
    PYBG
    		99.87%
    PYBG acts as a versatile precursor to be facilely conjugated with various fluorescent dyes through ‘Click chemistry’ and Sonogashira coupling reactions. PYBG is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
    PYBG
  • HY-D1812
    Vari Fluor 488-dUTP
    		≥99.0%
    Vari Fluor 488-dUTP (VF 488-dUTP) can replace its natural counterpart, dTTP, by enzymatic incorporation into DNA/cDNA. Vari Fluor 555-dUTP can be used to produce biotinylated DNA probes in a variety of assay applications.
    Vari Fluor 488-dUTP
  • HY-P4419A
    Boc-Asp(OBzl)-Pro-Arg-AMC acetate
    		98.77%
    Boc-Asp(OBzl)-Pro-Arg-AMC acetate is a fluorogenic substrate for the determination of protease activity. Boc-Asp(OBzl)-Pro-Arg-AMC acetate undergoes hydrolysis and releases the fluorescent product 7-amino-4-methylcoumarin (AMC). AMC is fluorescent under UV light and can emit a fluorescent signal.
    Boc-Asp(OBzl)-Pro-Arg-AMC acetate
  • HY-135172
    Suc-Leu-Tyr-AMC
    		98.05%
    Suc-Leu-Tyr-AMC is a fluorometric peptide substrate for µ-calpain and m-calpain. The excitation wavelength is 360 nm and the emission wavelength is 460 nm.
    Suc-Leu-Tyr-AMC
  • HY-119791
    NBD-amine
    NBD-amine is a fluorogenic amine. NBD-amine displays the property of fluorescing weakly in water and strongly in organic solvents, membranes or hydrophobic environments.
    NBD-amine
Cat. No. Product Name / Synonyms Application Reactivity