2. Signaling Pathways
  3. Others
  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-15631
    Hoechst 33258 analog 6
    		99.63%
    Hoechst 33258 analog 6 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution (Ex/Em = 356/451 nm).
    Hoechst 33258 analog 6
  • HY-W127832
    Ru(dpp)3(PF6)2
    		98.20%
    Ru(dpp)3(PF6)2 is a complex containing metallic ruthenium (Ru) and is a chemiluminescent oxygen probe. [Ru(dpp)3]2+ is a luminescent substance that can undergo metal-to-ligand charge transfer (MLCT), and fluorescence will appear during the transfer of Ru to the ligand. At the same time, the fluorescence of Ru(dpp)3(PF6)2 is effectively quenched by molecular oxygen, so it is widely used in biosensors, environmental monitoring, and materials science. The property of Ru(dpp)3(PF6)2, which oxidizes Ru2+ to Ru3+, has also been used to design and construct solid-state light-emitting electrochemical cells.
    Ru(dpp)3(PF6)2
  • HY-D2339
    H-FluNox
    		99.53%
    H-FluNox is a selective fluorescent probe that senses labile heme using biomimetic N-oxide deoxygenation to trigger fluorescence. H-FluNox is more than 100-fold selective for labile heme over Fe(II), enabling the discrimination of labile heme from labile Fe(II) pools in living cells. H-FluNox is sensitive enough to detect the subcellular labile heme. (λex=490 nm, λem=535 nm)
    H-FluNox
  • HY-W923198
    Fluorescein O-methacrylate
    Fluorescein O-methacrylate (Methacryloyloxy fluorescein) is a pH-sensitive dye featuring a fluorescent monomer, characterized by an excitation spectrum at 490 nm and an emission spectrum at 520 nm. With fluorescein serving as an indicator that possesses minimal negative charges, it exhibits properties such as biocompatibility, non-toxicity, and excellent dispersion in aqueous solutions.
    Fluorescein O-methacrylate
  • HY-160870
    Firefly luciferase-IN-3
    Firefly luciferase-IN-3 (CID: 17087944) is an inhibitor of ATP-dependent luciferase (Firefly luciferase) and has nM-level inhibitory activity against NanoLuc (pIC50=7.5).
    Firefly luciferase-IN-3
  • HY-D1482
    Sulfo-Cy5-tetrazine
    Sulfo Cy5-tetrazine is a tetrazine-coupled Cy5 (HY-D0821) derivative dye with fluorescence properties similar to CY5 (Ex/Em=633/647 nm). Sulfo-Cy5-tetrazine is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.
    Sulfo-Cy5-tetrazine
  • HY-116862
    Dibenzylfluorescein
    		99.54%
    Dibenzylfluorescein (DBF) is a fluorogenic probe (Fluoresecent dye) that acts as a substrate for specific cytochrome P450 (CYP) isoforms, including CYP3A4, CYP2C8, CYP2C9, CYP2C19, and aromatase (CYP19). Dibenzylfluorescein is typically used near its Km value of 0.87-1.9 μM (Ex=485 nm，Em=535 nm). Dibenzylfluorescein is used to detect changes in CYP catalytic activity caused by drugs or disease.
    Dibenzylfluorescein
  • HY-15632
    para-iodoHoechst 33258
    		98.94%
    para-iodoHoechst 33258 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
    para-iodoHoechst 33258
  • HY-D2762
    18:1 PE TopFluor AF 594 triammonium
    18:1 PE TopFluor AF 594 triammonium is a fluorescently labeled phospholipid probe. 18:1 PE TopFluor AF 594 triammonium can be used as a fluorescent tracer for lipid nanoparticles (LNPs). 18:1 PE TopFluor AF 594 triammonium is promising for research of drug delivery systems.
    18:1 PE TopFluor AF 594 triammonium
  • HY-D0921
    Heptamethine cyanine dye-1
    Heptamethine cyanine dye-1 is a near-infrared cyanine dye for fluorescence imaging in biological systems.
    Heptamethine cyanine dye-1
  • HY-P1004A
    Luciferase, firefly
    		≥98.0%
    Luciferase, firefly is the light-emitting enzyme responsible for the bioluminescence of fireflies and click beetles.
    Luciferase, firefly
  • HY-D0219A
    Thymol Blue sodium
    Thymol Blue sodium is an acid-base indicator used to indicate changes in pH. Thymol Blue sodium fades from red to yellow at pH 1.2 to 2.8 and from yellow to blue at pH 8.0 to 9.6. Quantitative detection is achieved through the absorption peak shift (435 nm/596 nm) of the UV-visible spectrum. Thymol blue sodium can be fixed in a silica gel matrix through sol-gel technology to form a solid-state sensor for in-situ pH measurement in the marine environment and acid-base monitoring in the biomedical field.
    Thymol Blue sodium
  • HY-101901
    Oxazine 1 perchlorate
    		≥99.0%
    Oxazine 1 perchlorate is a symmetric cationic dye (λex=653 nm, λem=666 nm).
    Oxazine 1 perchlorate
  • HY-D1029A
    Biotin-11-dUTP trisodium
    		≥99.0%
    Biotin-11-dUTP trisodium is a fluorescent substitute for dTTP.
    Biotin-11-dUTP trisodium
  • HY-121930
    Coumarin 6H
    		99.63%
    Coumarin 6H, a Coumarin (Coumarin (HY-N0709)) derivative, is a laser dye. The fluorescence emission of Coumarin 6H can be enhanced by hydrogen bonding interactions.
    Coumarin 6H
  • HY-W777012
    Acridinium C2 NHS ester
    Acridinium C2 NHS ester is a chemiluminescent label that is active for the development of protein and nucleic acid probes. Acridinium C2 NHS ester can be used in bioanalysis and diagnostics to provide highly sensitive detection solutions. Acridinium C2 NHS ester is widely used in medical research, especially in the monitoring and analysis of biomolecules.
    Acridinium C2 NHS ester
  • HY-D0957
    Ethyl Violet
    Ethyl Violet is a triarylmethane dye. Ethyl Violet is a useful reagent for the determination of anionic surfactants.
    Ethyl Violet
  • HY-D1223
    AF488 NHS ester diTEA
    AF488 NHS ester diTEA is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells. AF488 exhibits the λem and λex wavelength of 520 nm and 470 nm, respectively.
    AF488 NHS ester diTEA
  • HY-W351339
    BAPTA-TMFM
    BAPTA-TMFM is a fluorescent chelating indicator used to study the role of cytosolic free calcium.
    BAPTA-TMFM
  • HY-D1396
    Br-DAPI
    		≥98.0%
    Br-DAPI is a marker dye in DAPI series. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells. Storage: Keep away from light.
    Br-DAPI
Cat. No. Product Name / Synonyms Application Reactivity