2. Signaling Pathways
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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-139014
    N-ε-propargyloxycarbonyl-L-lysine
    		≥98.0%
    N-ε-propargyloxycarbonyl-L-lysine (H-L-Lys(Poc)-OH) is a lysine-based unnatural amino acid (UAA). N-ε-propargyloxycarbonyl-L-lysine is widely used for bio-conjugation of fluorescent probes in diverse organisms from E. coli to mammalian cells even in animals. N-ε-propargyloxycarbonyl-L-lysine is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
    N-ε-propargyloxycarbonyl-L-lysine
  • HY-W248594
    IR 813 tosylate
    		99.47%
    IR 813 tosylate is a near-infrared (NIR) fluorescent dye and can be used for visualizing regional lymph nodes in mice.
    IR 813 tosylate
  • HY-D1732
    FITC-hyodeoxycholic acid
    		98.94%
    FITC-hyodeoxycholic acid is a hyodeoxycholic acid labeled with FITC, which can be used to study the mechanism of action of hyodeoxycholic acid.
    FITC-hyodeoxycholic acid
  • HY-D2208
    SYTM Green
    SYTM Green is a permeable DNA dye that fluoresces green by binding to dsDNA (Ex/Em=503/530 nm). SYTM Green can also be used to stain bacteria, including Gram-positive and Gram-negative bacteria. Note: SYTM Green cannot penetrate living cells, and this product is equivalent to (Ex/Em=488/530 nm).
    SYTM Green
  • HY-136936
    PYBG-BODIPY
    		98.27%
    PYBG-BODIPY is a dye and has a role as a fluorochrome. PYBG-BODIPY specifically and efficiently labels the target genetically encoded SNAP-tags in live cells.
    PYBG-BODIPY
  • HY-W103047
    1-Pyrenebutyric acid
    1-Pyrenebutyric acid is a fluorescence probe that can be used in fluorescence determination of DNA. 1-Pyrenebutyric acid can be used as a linker for biomolecules to form a self-assembled monolayer on grapheme.
    1-Pyrenebutyric acid
  • HY-NP043
    Avidin-Cy3
    Avidin-Cy3 is Cy3-labeled Avidin. Avidin has excellent affinity with biotin and is often used in combination with biotin for immunoassays to detect the location of antigens in tissues.
    Avidin-Cy3
  • HY-D1590
    BODIPY Green 8-P2M
    		99.21%
    ODIPY Green 8-P2M is a novel thiol-reactive fluorescence probe based on the BODIPY fluorophore, the fluorescence is strongly quenched by d-PeT and then can be restored after reaction with thiol, resulting in an extremely high signal-to-noise ratio. ODIPY Green 8-P2M can be useful for detecting extremely low concentrations of protein in the gel after SDS-PAGE.
    BODIPY Green 8-P2M
  • HY-D0943
    Nuclear Fast Red
    Nuclear Fast Red, an anthraquinone dye, is commonly used in conjunction with an excess of aluminum ions as a red nuclear counterstain. Nuclear fast red has been used as a histochemical and colorimetric reagent for calcium. Nuclear fast red as highly sensitive "off/on" fluorescent probe for detecting guanine.
    Nuclear Fast Red
  • HY-W008292
    Carbostyril 124
    		99.72%
    Carbostyril 124 is a sensitising chromophore. Carbostyril 124 is a reasonably effective organic sensitizer for a proximate bound terbium ion.
    Carbostyril 124
  • HY-D1880
    Sulfo-Cy5 hydrazide
    Sulfo-Cy5 hydrazide is a fluorescent dye containing a hydrazide functionality (Ex/Em=646/662 nm).
    Sulfo-Cy5 hydrazide
  • HY-D0165
    Naphthoresorcinol
    		99.60%
    Naphthoresorcinol (1,3-Dihydroxynaphthalene) is a fluorescent dye (λex=330 nm, λem=380 nm) that can react with the NPPD (a tracer) and concentrated HCl and develop a red color. Naphthoresorcinol could be used as a background electrolyte (BGE) to determine the carbohydrates.
    Naphthoresorcinol
  • HY-W011618
    N-(9-Acridinyl)maleimide
    		99.97%
    N-(9-Acridinyl)maleimide is a maleimide type fluorescent thiol reagent. N-(9-Acridinyl)maleimide shows no substantial fluorescence but its coupling products with thiol compounds exhibit strong blue fluorescence. N-(9-Acridinyl)maleimide is used for fluorometrical analysis of cysteine and glutathione.
    N-(9-Acridinyl)maleimide
  • HY-147309
    16-Azidohexadecanoic acid
    		≥98.0%
    16-Azidohexadecanoic acid, a synthetic fatty acid, can be used as a modification marker for nucleotides and a molecular probe for fatty acid metabolism. 16-Azidohexadecanoic acid is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    16-Azidohexadecanoic acid
  • HY-P3722A
    Mca-PLAQAV-Dpa-RSSSR-NH2 TFA
    		98.43%
    Mca-PLAQAV-Dpa-RSSSR-NH2 is a fluorescent peptide and as one of fluorescent substrates of TNF-α converting enzyme (TACE): ADAM17, ADAM 9 and ADAM 10. Mca-PLAQAV-Dpa-RSSSR-NH2 is a substrate based on fluorescence resonance energy transfer, and its activity can be determined by the change of fluorescence intensity during pyrolysis.
    Mca-PLAQAV-Dpa-RSSSR-NH2 TFA
  • HY-D1254
    Trx-red
    Trx-red (NBL-SS perchlorate) is a red-emitting fluorescent probe derivatized from the nile blue fluorophore. Trx-red is used for selectively imaging thioredoxin (Trx) in live cells and in vivo (λex=615 nm, λem=661 nm).
    Trx-red
  • HY-133876
    4-Di-16-ASP
    		99.76%
    4-Di-16-ASP is a green fluorescent membrane dye. 4-Di-16-ASP is a lipophilic aminostyryl probe with a broad emission spectrum (can be detected with green, orange or even red filters). It is commonly used for neuronal membrane tracing (it diffuses faster than DiO).
    4-Di-16-ASP
  • HY-136248
    Cyanine 3 Tyramide
    Cyanine 3 Tyramide (Tyramide-Cy3), an orange fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids.
    Cyanine 3 Tyramide
  • HY-158082H
    TRITC-dextran, MW 2000000
    TRITC-dextran, MW 2000000 (Tetramethyl rhodamine isothiocyanate glucan, MW 2000000) is a fluorescent dye, with the molecular weight of 2000 kD. TRITC-dextran, MW 2000000 exhibits an excitation wavelength of 555 nm. TRITC-dextran, MW 2000000 is follicular and vessel penetrate, which is dependent on the molecular weight. TRITC-dextran, MW 2000000 is utilized in drug delivery for the stability of TRITC over a wide pH range (i.e. pH 2–11) and resistance to photo-bleaching.
    TRITC-dextran, MW 2000000
  • HY-D1323
    Cyanine5.5 maleimide chloride
    		98.09%
    Cyanine5.5 maleimide chloride is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance.
    Cyanine5.5 maleimide chloride
Cat. No. Product Name / Synonyms Application Reactivity