2. Signaling Pathways
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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D0785
    NBD-F
    		98.87%
    NBD-F (4-Fluoro-7-nitrobenzofurazan) is a pro-fluorescent reagent which is developed for amino acid analysis. NBD-F reacts with primary or secondary amines to produce a fluorescent product and used for analysis of amino acids and low molecular weight amines.
    NBD-F
  • HY-W116606
    Coumarin boronic acid
    		99.93%
    Coumarin boronic acid is a fluorescent probe. The excitation and emission wavelengths of coumarin boronic acid are set to 360 nm and 430 nm, respectively. Coumarin boronic acid can be used to monitor the formation of amino acid and protein hydroxyl peroxides in real time, which is beneficial for understanding the mechanisms of oxidative stress and protein post-translational modification.
    Coumarin boronic acid
  • HY-D1255
    Sel-green
    		99.98%
    Sel-green, a selective selenol fluorescent probe, is applied to quantify the Sec content in the selenoenzyme thioredoxin reductase and image endogenous Sec in live HepG2 cells.
    Sel-green
  • HY-D1613
    BODIPY FL C5-HPC
    		99.43%
    BODIPY FL C5-HPC is a lipophilic green fluorescent dye (Ex/Em: 500/510 nm).
    BODIPY FL C5-HPC
  • HY-D1275
    CAY10731
    CAY10731 (compound 3) is a highly selective fluorescent probe for detection of hydrogen sulfide (H2S). CAY10731 is used to monitor exo- and endogenous H2S in both cancer and normal cells. CAY10731 is applied for imaging of H2S in living tissues at variable depths and in nematodes.
    CAY10731
  • HY-NP035
    Ovalbumin-FITC
    Ovalbumin-FITC is an ovalbumin conjugated to FITC (Ex=495 nm; Em=525 nm). Ovalbumin-FITC can be internalized by cells via endocytosis.
    Ovalbumin-FITC
  • HY-126994
    6′-Amino-D-luciferin
    		98.01%
    6’-Amino-D-luciferin is a derivative of D-luciferin (HY-12591A) with an amino group replacing the 6-hydroxyl group. 6’-Amino-D-luciferin is a natural substrate of luciferase (Luc) and can be used for IVIS imaging in cell/animal experiments[1][2][3].
    6′-Amino-D-luciferin
  • HY-130027
    HKOCl-4
    HKOCl-4 (BXY2142) is a rhodol-based yellow fluorescent probe for the detection of hypochlorous acid with excellent sensitivity and selectivity. HKOCl-4 has longer absorption wavelength and better pH stability compared with fluorescein-based probes. Ex: 530 nm; Em 557 nm.
    HKOCl-4
  • HY-W013967
    1,6-Diphenylhexa-1,3,5-triene
    		98.26%
    1,6-Diphenylhexa-1,3,5-triene is a fluorescent probe.
    1,6-Diphenylhexa-1,3,5-triene
  • HY-D0202
    Arsenazo III
    Arsenazo III is a dye useful for detection of micromolar concentrations of ionized Ca2+. Arsenazo III can be used for determination of thorium, zirconium, uranium and rare earth elements.
    Arsenazo III
  • HY-110128
    Sulfidefluor 7-AM
    		≥99.0%
    Sulfidefluor 7-AM is a stable hydrogen sulphide (H2S) fluorescent probe. Sulfidefluor 7-AM is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Sulfidefluor 7-AM
  • HY-D1051
    Cy3-N3
    Cy3-N3 is a Cy3-azide fluorescent dye used to label for protein and nucleic acid. Cy3-N3 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Cy3-N3
  • HY-158742
    SBB-Analogue (GL13) Biotin
    		99.30%
    SBB-Analogue (GL13) Biotin (GL13; SBB-A-B) is composed of a Sudan Black B (SBB; HY-D0213) derivative conjugated to biotin. SBB-Analogue (GL13) Biotin detects robustly senescent cells. SBB-Analogue (GL13) Biotin is deprived of the false positive staining disadvantages of SA-β-gal due to serum starvation and cell confluency. SBB-Analogue (GL13) Biotin can be used in flow cytometry and immunofluorescence analysis, etc.
    SBB-Analogue (GL13) Biotin
  • HY-134654
    MRTX849 analog 24
    		99.60%
    MRTX849 analog 24 is an alkyne-containing click probe analogue of KRAS G12C inhibitor MRTX849, which is used to research MRTX849 function.
    MRTX849 analog 24
  • HY-W040681
    7-Dimethylaminocoumarin-4-acetic acid
    		98.05%
    7-Dimethylaminocoumarin-4-acetic acid is a fluorescent probe.
    7-Dimethylaminocoumarin-4-acetic acid
  • HY-134435
    Z-Arg-AMC hydrochloride
    		99.2%
    Z-Arg-AMC hydrochloride is a fluorogenic substrate for trypsin and papain. Z-Arg-AMC hydrochloride can be used to research activity of trypsin (Ex/Em=355/460 nm).
    Z-Arg-AMC hydrochloride
  • HY-D1266
    RB-OPD
    RB-OPD (NO-red) is a o-phenylenediamine (OPD)-locked rhodamine nitric oxide (NO) fluorescent probe with great sensitivity and selectivity (λex=550 nm, λem=590 nm).
    RB-OPD
  • HY-N0917
    6''-O-Malonylgenistin
    		99.24%
    6''-O-Malonylgenistin(Malonylgenistin) is an isoflavone derivative.
    6''-O-Malonylgenistin
  • HY-D1390A
    Sulfo-Cy5-Mal
    Sulfo-Cy5-Mal is a fluorescent dye with the form of active ester that reacts with thiol groups in proteins to form stable conjugates. Sulfo-Cy5-Mal that can be used to label protein.
    Sulfo-Cy5-Mal
  • HY-D1565
    Cy7.5 maleimide
    Cy7.5 maleimide is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance.
    Cy7.5 maleimide
Cat. No. Product Name / Synonyms Application Reactivity