2. Signaling Pathways
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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D1831
    Vari Fluor 620 TSA（200×）
    Vari Fluor 620 TSA（200×） (VF 620 Tyramide) is a fluorescent probe belonging to the Vari Fluor TSA series. Vari Fluor TSA series fluorescent probes can be used for high-density in situ labeling of target antigens using horseradish peroxidase (HRP) assays, which are widely used in ELISA, IF, IHC and other techniques with high detection sensitivity.
    Vari Fluor 620 TSA（200×）
  • HY-D1599
    Rhodamine 6G hydrazide
    		98.05%
    Rhodamine 6G hydrazide (R6GH) is a fluorescent dye. Rhodamine 6G hydrazide can be used in selective colorimetric and fluorescent sensing.
    Rhodamine 6G hydrazide
  • HY-D1685
    BZiPAR
    BZiPAR is a fluorescent probe. BZiPAR also is a substrate of trypsin that becomes fluorescent after cleavage by the protease.
    BZiPAR
  • HY-D1151
    HKOH-1
    		98.13%
    HKOH-1 is a highly sensitive green fluorescent probe for the specific detection of ·OH in living cells with a maximum excitation wavelength and emission wavelength of 500 nm and 520 nm, respectively.
    HKOH-1
  • HY-D1800
    Vari Fluor 680 SE
    Vari Fluor 680 SE (VF 680 SE) is a dye marker of the Vari Fluor SE series (Ex/Em=680 nm/700 nm). The Vari Fluor SE series of dyes are a class of fluorescent dyes containing NHS ester groups used to label free amines (-NHX) on antibodies, proteins, peptides, amine-modified oligonucleotides and other biomolecules.
    Vari Fluor 680 SE
  • HY-P4319
    Boc-Glu-Lys-Lys-AMC
    		99.97%
    Boc-Glu-Lys-Lys-AMC is a sensitive fluorogenic substrate for urokinase-activated plasmin.
    Boc-Glu-Lys-Lys-AMC
  • HY-113862
    PHOME
    		≥99.0%
    PHOME is a fluorogenic substrate for sEH. sEH can hydrolyze the epoxy ring in the PHOME substrate. PHOME can be used for fluorescent epoxide hydrolase assay.
    PHOME
  • HY-W073524
    DFAME
    		98.58%
    DFAME is a red fluorophore (Ex=508 nm, Em=641 nm). Beetroot and Corn are dimeric fluorogenic RNA aptamers that can bind to DFAME to form Beetroot-DFAME (Kd=460nM) and Corn-DFAME (Kd= 3600nM). Beetroot-DFAME (Kd=460nM) and Corn-DFAME can be used to form RNA assemblies in living cells. Creating RNA assemblies can be used for the study of RNA Nanostructures. DNA/RNA Nanostructures would be useful in cell and gene therapy (CGT) research.
    DFAME
  • HY-126395
    Patent Blue V calcium salt
    		99.99%
    Patent Blue V calcium salt is a triarylmethane dye used for tissue staining and lymphatic tracing, mainly applied by topical injection or eye drops. Patent Blue V calcium salt has affinity for specific tissues (such as corneal endothelium, lymphatic system), and stains the target structure by adsorption or binding, assisting in precise operation during surgery. Patent Blue V calcium salt is mainly used in ophthalmic surgery (such as graft staining for Descemet's membrane endothelial keratoplasty) and lymphatic drainage localization for sentinel lymph node biopsy of tumors.
    Patent Blue V calcium salt
  • HY-W039938
    4-Nitrophenyl β-D-xylopyranoside
    		99.85%
    4-Nitrophenyl β-D-xylopyranoside is a chromogenic β-xylosidase substrate. 4-Nitrophenyl β-D-xylopyranoside can be used to test β-xylosidase activity.
    4-Nitrophenyl β-D-xylopyranoside
  • HY-W040226
    Indigo carmine
    		≥98.0%
    Indigo carmine is an efficient reagent for the determination of ozone by chemlluminescence (CL).
    Indigo carmine
  • HY-D1353
    LipidGreen 2
    		98.94%
    LipidGreen 2 is a second generation small molecule probe for lipid imaging. LipidGreen 2 has a better fluorescence signal compared with the previous LipidGreen, and selectively stains neutral lipids in cells and fat deposits in live zebrafish.
    LipidGreen 2
  • HY-D0194
    5(6)-Carboxy-2',7'-dichlorofluorescein
    		99.32%
    5(6)-Carboxy-2',7'-dichlorofluorescein is an ideal substrate for MRP2 vesicular transport assay, with excellent detection and transport properties.
    5(6)-Carboxy-2',7'-dichlorofluorescein
  • HY-D1833
    Vari Fluor 680 TSA (200×)
    Vari Fluor 680 TSA (200×) (VF 680 Tyramide) is a fluorescent probe belonging to the Vari Fluor TSA series. Vari Fluor TSA series fluorescent probes can be used for high-density in situ labeling of target antigens using horseradish peroxidase (HRP) assays, which are widely used in ELISA, IF, IHC and other techniques with high detection sensitivity.
    Vari Fluor 680 TSA (200×)
  • HY-156404
    PM-1
    		99.04%
    PM-1, a derivative of Thioflavin-T (ThT; HY-D0218), is a small but highly specific plasma membrane (PM) fluorescent dye for specific and long-time membrane imaging of living and fixed cells. PM-1 is embedded directly into the cell membrane and exhibits a very long retention time on the plasma membrane with a half-life of approximately 15 h. PM-1 can be used in combination with protein labeling probes to study ectodomain shedding and endocytosis processes of cell surface proteins.
    PM-1
  • HY-W027968
    Coumarin-3-carboxylic Acid
    		99.93%
    Coumarin-3-carboxylic acid (2- Oxochromene-3-carboxylic acid) is an important initial compound for the synthesis of coumarins which are well known natural products for their diverse biological activities. Lanthanide complexes of Coumarin-3-carboxylic acid exhibit antiproliferative activity towards K-562 cell line.
    Coumarin-3-carboxylic Acid
  • HY-W181026
    KLF10-IN-1
    		99.84%
    KLF10-IN-1 (#48-15) is a KLF10 inhibitor with an IC50 value of 40 μM for KLF10 reporter gene. KLF10-IN-1 can inhibit KLF10-DNA binding and transcriptional activity, block the conversion of CD4+CD25 T cells to CD4+CD25+T regulatory cells, and inhibit the expression of KLF10 target genes. KLF10-IN-1 can be used as a useful mechanistic probe to study KLF10-mediated effects and T regulatory cell biology.
    KLF10-IN-1
  • HY-D0721
    6-CFDA
    		98.09%
    6-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase. CF containing portion contains an additional negative charge so that it is better retained in cells, compared to fluorescein dyes.
    6-CFDA
  • HY-107869
    Isoindigo
    		99.12%
    Isoindigo is the acceptor of donor-acceptor (D-A) conjugated polymer, as well as an Indigo series dye. Isoindigo equips two lactam rings and strong electron-withdrawing character. Isoindigo can be used for dyeing and pigment preparation. Isoindigo shows high mobility and good ambient stability in FETs.
    Isoindigo
  • HY-NP055
    Ovalbumin-Cy5
    Ovalbumin-Cy5 is Cy5-labeled Ovalbumin (OVA).
    Ovalbumin-Cy5
Cat. No. Product Name / Synonyms Application Reactivity