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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D1429
    ER-Tracker Blue-White DPX
    ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide (HY-15206), highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K+ channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining cells after fixation.
    ER-Tracker Blue-White DPX
  • HY-47176
    QTZ
    QTZ is a bioluminescence agent for in vivo imaging. QTZ has red-shifted emission and yields very little background. QTZ is a coelenterazine analog with the 4-quinolinyl substitution at the C8 position of the imidazopyrazinone core.
    QTZ
  • HY-D1553
    BODIPY-C12
    		99.84%
    BODIPY-C12 is a boron-dipyrromethene derivative containing a lipophilic tail (excitation 490 nm). BODIPY-C12 is a hydrophobic molecular and sensitivity to the surrounding environment and can be used to quantify viscosity, critical micelle concentration, and critical micelle temperature.
    BODIPY-C12
  • HY-140296
    BDP FL DBCO
    		98.86%
    BDP FL DBCO is a photostable boron dipyrromethene used in the FAM (fluorescein) channel, with compatible property for fluorescein filters. BDP FL DBCO contains DBCO (azadibenzocyclooctyne) groups that enable copper-free catalyzed click chemistry reactions with azides. λex/λem= 503 nm/512 nm.
    BDP FL DBCO
  • HY-D0211
    Phenolphthalein
    		99.92%
    Phenolphthalein is an acid-base indicator that is colorless in an acidic environment and pink in an alkaline environment. Phenolphthalein is genotoxic and potentially carcinogenic to mice. In addition, phenolphthalein is also a blood indicator.
    Phenolphthalein
  • HY-101937B
    L-ANAP hydrochloride
    		99.89%
    L-ANAP hydrochloride is a genetically encodable and polarity-sensitive fluorescent unnatural amino acid (Uaa).
    L-ANAP hydrochloride
  • HY-114879
    DDAO
    		98.43%
    DDAO is a promising near-infrared (NIR) red fluorescent probewith tunable excitation wavelength (600-650nm) and longemission wavelength(λem=656nm). DDAO can de desiged for detection of the activities of different enzymes such asβ-galactosidase,sulfatase, proteinphosphatase2A,carboxylesterase 2, humanalbumin andesterases.
    DDAO
  • HY-P2377
    Ac-YVAD-AFC
    		99.56%
    Ac-YVAD-AFC is the the fluorometric peptide substrate of caspase-1, with λ excitation of 400 nm and λ emission of 505 nm.
    Ac-YVAD-AFC
  • HY-D1176
    Calcium Green 1AM
    Calcium Green 1AM is a cell-permeant fluorescent calcium indicator (Excitation 506 nm; Emission 531 nm). Calcium Green 1AM is converted to the fluorescent calcium indicator by intracellular esterases.
    Calcium Green 1AM
  • HY-D1694
    LysoTracker Yellow HCK 123
    		98.51%
    LysoTracker Yellow HCK 123 is a potent yellow membrane-permeable fluorescent probe. LysoTracker Yellow HCK 123 is a weakly basic amine that selectively accumulates in cellular compartments with low luminal pH.
    LysoTracker Yellow HCK 123
  • HY-D1296
    Green DND-26
    		98.95%
    Green DND-26 is a green fluorescently labeled lysosomal probe with a maximum excitation/emission wavelength of 504/511 nm. The structure is composed of a fluorescein group and linked weak bases, which can freely cross the cell membrane and generally gather on spherical organelles. Green DND-26 is suitable for observing the internal biosynthesis and related pathogenesis of lysosomes.
    Green DND-26
  • HY-126831
    SBFI-AM
    		98.0%
    SBFI-AM is a Na+ selective fluorescent indicator. SBFI-AM shows selectivity for Na+ over K+.
    SBFI-AM
  • HY-15938
    5-FAM SE
    5-FAM SE is a single isomer, is a fluorescent labeling reagent used for labeling peptides, proteins and nucleotides. 5-FAM SE can react with amines and can yield stable amine conjugates.
    5-FAM SE
  • HY-D0723
    5(6)-TAMRA SE
    5(6)-TAMRA SE is a fluorescent dye that emits red fluorescence. 5(6)-TAMRA SE binds to oligonucleotides and is used in DNA sequencing. 5(6)-TAMRA SE can be used in cancer research (Ex/Em = 565/580 nm).
    5(6)-TAMRA SE
  • HY-D0982
    Zinquin
    		99.00%
    Zinquin is a fluorescent sensor and used to observe reactive Zn2+.λex/λem=368/490 nm.
    Zinquin
  • HY-D1375
    Sulfo-Cy5 amine
    		≥98.0%
    Sulfo-Cy5 amine is a dye derivative of Cyanine 5 (Cy5) (HY-D0821) bearing an amine group. The sulfonate ion increases the water solubility of the compound, making it suitable for use in aqueous solutions. Cy5 is a near-infrared fluorescent dye commonly used in biolabeling and cell imaging. The amine functionality of Sulfo-Cy5 amine can react with carboxyl groups to form covalent bonds. Sulfo-Cy5 amine can bind to biomolecules such as proteins and antibodies to track their location and dynamic changes in biological samples.
    Sulfo-Cy5 amine
  • HY-W127770
    Pararosaniline hydrochloride
    		98.28%
    Pararosaniline hydrochloride (Basic red 9) is a pH-responsive basic dye, as a biological stain to track certain proteins. The pH of the acidified Pararosaniline hydrochloride reagent has a significant effect on the color and the maximum absorption wavelength (λmax) of the reaction system, with its optimum pH 0.48 and a λmax at 549 nm. Pararosaniline hydrochloride is also a strong modifier of RNA splicing. Pararosaniline hydrochloride has been used in the analysis of SO2 and formaldehyde and staining of bacteria or other organisms. Pararosaniline hydrochloride is extensively used in industries like textile, printing, paper, cosmetic, and leather.
    Pararosaniline hydrochloride
  • HY-D1683
    NBD-PE
    		99.0%
    NBD-PE is an effective lipid fluorescent probe (Excitation/Emission: 465/535 nm; Color: Green). NBD-PE offers a wide array of applications in membrane and cell biology.
    NBD-PE
  • HY-D0232
    Brilliant Blue R250
    Brilliant blue R250 (Brilliant Blue R), an anionic dye, is the most popular stain to detect proteins resolved in SDS-PAGE gels.
    Brilliant Blue R250
  • HY-D0827
    Cy2
    		≥98.0%
    Cyanines are formally compounds with two nitrogen atoms linked by an odd number of methene units. 26 28 The nitrogen atoms are parts of the heterocyclic units (such as indole, benzoxazol, or benzothiazol) . The structures and optical properties of representative cyanine dyes used for in vivo imaging are presented. Cyanines are characterized by long wavelength, tunable absorption and emission, very high extinction coefficient (up to 300,000 M 1 cm 1), good water solubility, and relatively straightforward synthesis.
    Cy2
Cat. No. Product Name / Synonyms Application Reactivity