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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D1601
    N-Aminofluorescein
    		99.91%
    N-Aminofluorescein is a fluorescein hydrazide with spiro form, a highly selective and sensitive fluorescence probe for Cu2+. N-Aminofluorescein has no selective fluorescence response to other common metal ions, can be used for direct detection of Cu2+ in biological systems with λex/em=495/516 nm.
    N-Aminofluorescein
  • HY-12489
    Ponceau S
    		≥98.0%
    Ponceau S (Acid Red 112) is a non-specific protein dye commonly used as a stain for Western blot. Ponceau S is used in an acidic aqueous solution that is compatible with antibody-antigen binding and dyes the proteins on the membrane red.
    Ponceau S
  • HY-129096
    IDT307
    		99.76%
    IDT307, an analog of the organic cation MPP+, is a specific fluorescent substrate for DAT (fluorescent substrate APP+).
    IDT307
  • HY-D0169A
    Phenol Red sodium salt
    Phenol red (Phenolsulfonephthalein) sodium salt is a pH indicator dye. Phenol Red sodium salt is also an antagonist of rat P2X1R. Phenol Red sodium salt enhances the halogenating activity of myeloperoxidase. Phenol Red sodium salt shows a distinct color change from pink to yellow in a positive pH reaction.
    Phenol Red sodium salt
  • HY-D0807
    5-IAF
    5-IAF (5-Iodoacetamidofluorescein) is an idoacetamide derivate of fluoresceine. 5-IAF can be used as fluorescent probe that labels proteins and other molecules having free thiols (cysteine side chains).
    5-IAF
  • HY-B0389F
    Biotin-D-Glucose
    Biotin-D-Glucose is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells.
    Biotin-D-Glucose
  • HY-135415
    Cyanine5.5 NHS ester chloride
    Cyanine5.5 NHS ester chloride is a reactive dye for the labeling of proteins, and antibodies and small molecular compounds.
    Cyanine5.5 NHS ester chloride
  • HY-101937B
    L-ANAP hydrochloride
    		99.89%
    L-ANAP hydrochloride is a genetically encodable and polarity-sensitive fluorescent unnatural amino acid (Uaa).
    L-ANAP hydrochloride
  • HY-114879
    DDAO
    		98.43%
    DDAO is a promising near-infrared (NIR) red fluorescent probewith tunable excitation wavelength (600-650nm) and longemission wavelength(λem=656nm). DDAO can de desiged for detection of the activities of different enzymes such asβ-galactosidase,sulfatase, proteinphosphatase2A,carboxylesterase 2, humanalbumin andesterases.
    DDAO
  • HY-135368
    8pyDTZ
    		≥98.0%
    8pyDTZ is a pyridyl diphenylterazine (DTZ) analog and an ATP-independent pyridyl substrate of LumiLuc luciferase. 8pyDTZ exhibits spectrally shifted emission. 8pyDTZ has excellent biocompatibility and superior in vivo sensitivity. 8pyDTZ can be used for in vivo luminescence imaging.
    8pyDTZ
  • HY-B1539A
    Fuchsine base monohydrochloride
    		99.53%
    Fuchsine base (monohydrochloride) is a magenta dye, which is certified for use for acid-fast staining with carbol-fuchsin.
    Fuchsine base monohydrochloride
  • HY-D1261
    Naph-EA-mal
    Naph-EA-mal (Thiol-green 1) is a rapid detect and ultrafast turn-on thiol fluorescence probe for protein labeling and bioimaging. Naph-EA-mal (Thiol-green 1) can be used to detect thiols in living cells, label the protein thiols, quantify the concentration of total thiols in cell lysate, and determine the reversible protein thiols oxidation in fixed cells. Ex: 488 nM; Em: 540 nM.
    Naph-EA-mal
  • HY-D2119
    CQ-Lyso
    		98.95%
    CQ-Lyso is a lysosome-targeting chromenoquinoline. CQ-Lyso is a fluorescent probe for measuring the pH of lysosomes in living cells using only single wavelength excitation. CQ-Lyso can visualize the dynamic pH changes in lysosomes.
    CQ-Lyso
  • HY-D1320
    Cyanine5 azide chloride
    		99.63%
    Cyanine5 azide chloride is a potent fluorescent dye. Cyanine5 azide chloride can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). (λex=646 nm, λem=662 nm). It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
    Cyanine5 azide chloride
  • HY-D1429
    ER-Tracker Blue-White DPX
    ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide (HY-15206), highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K+ channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining cells after fixation.
    ER-Tracker Blue-White DPX
  • HY-15930A
    TMB dihydrochloride
    		99.83%
    TMB dihydrochloride is the dihydrochloride form of TMB (HY-15930). TMB dihydrochloride is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB dihydrochloride can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB dihydrochloride can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922.
    TMB dihydrochloride
  • HY-W879007
    ICG-azide
    		99.66%
    ICG-azide, a near infrared (NIR) fluorescent dye, is a copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) reagent. ICG-azide can be readily incorporated into dye-stabilized nanoemulsions and facilitate the attachment of targeting ligands via click-chemistry in a simple, scalable, and reproducible reaction.
    ICG-azide
  • HY-D0784
    5-ROX
    		98.68%
    5-ROX (5-Carboxy-X-rhodamine), a rhodamine dye, exhibits strong fluorescence property in aqueous buffer with the λexit of 580 nm (ε=3.6×104 M-1 cm-1), and λemit of 604 nm (=0.94).
    5-ROX
  • HY-108715A
    RT-AM
    		98.07%
    RT-AM is a pro-agent real thiol. Real Thiol is a reversible reaction-based fluorescent probe which can quantitatively monitor the real-time glutathione dynamics in living cells.
    RT-AM
  • HY-D0935A
    4-Methylumbelliferyl-β-D-glucuronide hydrate
    		99.92%
    4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λex=362 nm, λem=445 nm). 4-Methylumbelliferyl-β-D-glucuronide hydrate has potential applications in detecting the activity of β-glucuronidase and the number of Escherichia coli.
    4-Methylumbelliferyl-β-D-glucuronide hydrate
Cat. No. Product Name / Synonyms Application Reactivity