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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D1261
    Naph-EA-mal
    Naph-EA-mal (Thiol-green 1) is a rapid detect and ultrafast turn-on thiol fluorescence probe for protein labeling and bioimaging. Naph-EA-mal (Thiol-green 1) can be used to detect thiols in living cells, label the protein thiols, quantify the concentration of total thiols in cell lysate, and determine the reversible protein thiols oxidation in fixed cells. Ex: 488 nM; Em: 540 nM.
    Naph-EA-mal
  • HY-D1601
    N-Aminofluorescein
    		99.91%
    N-Aminofluorescein is a fluorescein hydrazide with spiro form, a highly selective and sensitive fluorescence probe for Cu2+. N-Aminofluorescein has no selective fluorescence response to other common metal ions, can be used for direct detection of Cu2+ in biological systems with λex/em=495/516 nm. N-Aminofluorescein can be used to measure the concentration of copper ions in cells.
    N-Aminofluorescein
  • HY-P3236
    N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide
    		99.68%
    N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide (Ac-IEPD-pNA) is a colorimetric peptide substrate of granzyme B that allows accurate measurement of granzyme B activity.
    N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide
  • HY-D0817
    Rhodamine 110
    		99.23%
    Rhodamine 110 is a sensitive and selective substrate for assaying proteinases in solution or inside living cells. The excitation wavelength is 498 nm and the emission wavelength is 521 nm.
    Rhodamine 110
  • HY-D2348A
    ACE TFA
    		99.47%
    ACE TFA is a non-GFP fluorescent dye. ACE TFA can be conjugated with fluorescent RNA aptamers for RNA visualization studies in living cells.
    ACE TFA
  • HY-119323
    7-Azido-4-methylcoumarin
    		98.90%
    7-Azido-4-methylcoumarin is a fluorescent hydrogen sulfide (H2S) probe. 7-Azido-4-methylcoumarin serves as a highly sensitive assay for cystathionine β-synthase activity. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
    7-Azido-4-methylcoumarin
  • HY-W879007
    ICG-azide
    		99.66%
    ICG-azide, a near infrared (NIR) fluorescent dye, is a copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) reagent. ICG-azide can be readily incorporated into dye-stabilized nanoemulsions and facilitate the attachment of targeting ligands via click-chemistry in a simple, scalable, and reproducible reaction.
    ICG-azide
  • HY-D0935A
    4-Methylumbelliferyl-β-D-glucuronide hydrate
    		99.92%
    4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λex=362 nm, λem=445 nm). 4-Methylumbelliferyl-β-D-glucuronide hydrate has potential applications in detecting the activity of β-glucuronidase and the number of Escherichia coli.
    4-Methylumbelliferyl-β-D-glucuronide hydrate
  • HY-D1691
    BODIPY-581/591 NHS ester
    		98.67%
    BODIPY-581/591 NHS ester is a bright, red fluorescent dye (excitation: 581 nm; emission: 591 nm). BODIPY-581/591 NHS ester can be used for the labeling of amine containing biomolecules.
    BODIPY-581/591 NHS ester
  • HY-D1716
    S0456
    		99.44%
    S0456 is a raw material that plays a crucial role in the construction of the folate receptor-targeted near-infrared dye OTL 38 (HY-139579). S0456 is connected to the modified folic acid to form OTL 38, endowing OTL 38 with good optical properties, high affinity, and specificity. S0456 can be used in the research of tumor optical imaging agents.
    S0456
  • HY-101900
    Nile Blue A sulfate
    		≥98.0%
    Nile Blue A (Nile blue sulfate) is used to differentiate melanins and lipofuscins. It is also useful for staining fats and preparation of an amperometric glucose sensor.
    Nile Blue A sulfate
  • HY-D1080
    EDANS
    		99.90%
    EDANS (1,5-EDANS) is a novel and quenched fluorogenic substrate for assaying retroviral protease by resonance energy transfer (RET).
    EDANS
  • HY-139109
    IR-783
    		99.87%
    IR-783 (ADS 780WS) is a near-infrared (NIR) heptamethine cyanine fluorescent probe. IR-783 significantly inhibits tumour growth and induces apoptosis in MDA-MB-231 xenograft model. IR-783 can be used to study breast cancer.
    IR-783
  • HY-133852A
    FD-1080 free acid
    FD-1080 free acid is a fluorophore with both excitation and emission in the NIR-II region (Ex=1064 nm, Em=1080 nm). FD-1080 free acid can be used for in vivo imaging.
    FD-1080 free acid
  • HY-152073
    BETA-1
    		98.76%
    BETA-1 is the first twisted intramolecular charge transfer (TICT)-aggregation-induced emission (AIE) integration molecule. BETA-1 emits cyan fluorescence in lipid droplets (LDs) and red fluorescence in mitochondria. BETA-1 can be used for the simultaneous and dual-color imaging of LDs and mitochondria in vivo and in vitro.
    BETA-1
  • HY-129042
    Cibacron Blue 3G-A
    Cibacron Blue 3G-A is an anthraquinone dye, inhibits the R46 β-lactamase with a Ki value of 1.2 uM.
    Cibacron Blue 3G-A
  • HY-D0807
    5-IAF
    5-IAF (5-Iodoacetamidofluorescein) is an idoacetamide derivate of fluoresceine. 5-IAF can be used as fluorescent probe that labels proteins and other molecules having free thiols (cysteine side chains).
    5-IAF
  • HY-117401
    5-Dodecanoylaminofluorescein
    		99.32%
    5-Dodecanoylaminofluorescein, a lipophilic fluorescent probe, is a free-fatty-acid conjugate of fluorescein. 5-Dodecanoylaminofluorescein has been used in membrane fluidity studies and the determination of critical micelle concentration of detergents. 5-Dodecanoylaminofluorescein can be also used to synthesize hydrophobic nanospheres for drug delivery.
    5-Dodecanoylaminofluorescein
  • HY-137827
    4-Nitrophenyl β-D-Cellobioside
    		99.48%
    4-Nitrophenyl β-D-Cellobioside (p-Nitrophenyl β-D-cellobioside) is a cellotriose analog, a chromogenic substrate for the detection of cellulase activity. Exoglucanases, endoglucanases, and β-glucosidases hydrolyze 4-Nitrophenyl β-D-Cellobioside to yield p-nitrophenol (PNP).
    4-Nitrophenyl β-D-Cellobioside
  • HY-131008
    Fluorescein tyramide
    		≥99.0%
    Fluorescein tyramide is a green fluorescent reagent (λabs: 494 nm; λem: 517 nm). Fluorescein tyramide is widely used for tyramide signal amplification (TSA) with a low-abundance in IHC, ICC, in situ hybridization (FISH) and flow cytometry (FCM) applications.
    Fluorescein tyramide
Cat. No. Product Name / Synonyms Application Reactivity