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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D1742
    DRAQ5
    		Chemical
    DRAQ5 is a novel cell permeant and far red-fluorescing DNA probe. DRAQ5 excites at a wavelength of 647 nm, close to the Ex, and produces a fluorescence spectrum extending from 665 nm out to beyond 780 nm wavelengths. DRAQ5 fluorescence reflects cellular DNA content. DRAQ5 can be used in combination with FITC and RPE-labelled antibodies, without the need for fluorescence compensation.
    DRAQ5
  • HY-D0823
    CY3-SE
    Cy3-SE (Sulfo-Cy3 NHS ester; Sulfo Cyanine3 NHS ester) is a sulfonated cyanine dye-derived fluorescent labeling reagent with λex of about 515 nm and λem of about 568 nm. Cy3-SE can interact with the π-π stacking of biomolecules (e.g., nucleoside monophosphates, proteins), inhibiting the photoisomerization process and increasing the fluorescence quantum yield and lifetime.
    CY3-SE
  • HY-D2171A
    AF488 DBCO ditriethylamine
    AF488 DBCO ditriethylamine is the ditriethylamine salt form of AF488 DBCO (HY-D2171). AF488 DBCO ditriethylamine is a fluorescent dye that labels azide-containing biomolecules. DBCO is the bioorthogonal partner of azide that allows covalent coupling in the absence of copper. AF488 is a bright, photostable green fluorophore. AF488 exhibits the λem and λex wavelength of 520 nm and 470 nm, respectively.
    AF488 DBCO ditriethylamine
  • HY-137845
    4-Methylumbelliferyl β-D-galactopyranoside
    		99.06%
    4-Methylumbelliferyl β-D-galactopyranoside is a fluorescent substrate for β-galactosidase which, when cleaved, produces a water-soluble blue fluorescent coumarin fluorophore that can be detected using a fluoroenzymeter or fluorometer.
    4-Methylumbelliferyl β-D-galactopyranoside
  • HY-43520
    BODIPY-FL
    		99.98%
    BODIPY-FL is a green fluorescent dye that can be used to label probes or primers. BODIPY-FL fluorescence can be quenched after interacting with uniquely positioned guanine, making it useful for quantifying specific DNA or RNA molecules. BODIPY-FL-labeled sphingolipid analogs can be used to investigate sphingolipid internalization, transport, and endocytosis in mouse embryonic stem cells. BODIPY-FL-labeled monoterpenes can quickly penetrate bacteria, mammalian, and fungal cells, allowing for the detection of characteristics of a broad spectrum of Gram-positive and Gram-negative bacteria, as well as pathogenic fungi. The maximum absorption wavelength of BODIPY-FL is 505 nm, and the maximum emission wavelength is 513 nm.
    BODIPY-FL
  • HY-D0193
    Ponceau 4R
    		99.09%
    Ponceau 4R is a synthetic colorant that can be used for food coloring. Ponceau 4R is a strawberry red azo dye that can be used in a variety of foods, usually synthesized from aromatic hydrocarbons, and is stable to light, heat, and acids.
    Ponceau 4R
  • HY-D0259
    Erythrosine B
    		≥98.0%
    Erythrosine B is an artificial dye widely used in the food and textile industries. Erythrosine B is also a novel photosensitizer which has been used to develop animal models.
    Erythrosine B
  • HY-D0945
    Xylene Cyanol FF
    Xylene Cyanol FF is an acid triphenylmethane dye. Xylene Cyanol FF can be used for histochemical staining of hemoglobin peroxidase or as a tracking dye for DNA sequencing in electrophoresis. Xylene Cyanol FF will be catalyzed by Fe and Al to accelerate oxidation under the addition of double oxidant hydrogen peroxide and potassium periodate. Xylene Cyanol FF thus enables the spectrophotometric determination of Fe and Al in the solution to be tested.
    Xylene Cyanol FF
  • HY-D0367
    Fluorescent Brightener 28 (25% in water)
    Fluorescent brightener 28 is a fluorescent whitening agent commonly used in the padding process of the textile industry. Fluorescent brightener 28 is capable of staining polysaccharides such as cellulose, and when the plasma membrane ruptures, it also weakly stains the cytoplasm and strongly stains the cell nucleus. Additionally, Fluorescent brightener 28 can be utilized to detect intracellular chitin in living cells. Fluorescent Brightener 28 also is a visible light emitting diode (LED)-light sensitive photoinitiator for free radical photopolymerizations.
    Fluorescent Brightener 28 (25% in water)
  • HY-D1834
    Vari Fluor 640 TSA（200×）
    Vari Fluor 640 TSA（200×） (VF 640 Tyramide) is a fluorescent probe belonging to the Vari Fluor TSA series. Vari Fluor TSA series fluorescent probes can be used for high-density in situ labeling of target antigens using horseradish peroxidase (HRP) assays, which are widely used in ELISA, IF, IHC and other techniques with high detection sensitivity.
    Vari Fluor 640 TSA（200×）
  • HY-D1389
    Sulfo-CY-5.5 NHS ester tripotassium
    Sulfo-CY-5.5 NHS ester (Sulfo-Cyanine5.5 NHS ester) tripotassium is an amine-reactive ester of sulfonated far-red Cyanine5.5 fluorophore, which can be used to label antibodies, proteins, etc., as well as for in vivo NIR imaging.
    Sulfo-CY-5.5 NHS ester tripotassium
  • HY-120601
    Alizarin Red S sodium
    Alizarin Red S sodium is an anthraquinone derivative dye. When combined with cations such as calcium ions, the functional group of Alizarin Red S sodium can form a coordination bond with the cation through the oxygen atom to show orange-red fluorescence. Alizarin Red S sodium can be used for screening of calcium compounds in synovial fluid and detecting osteoblast differentiation, and can also be used for bone staining in mice. Excitation/emission wavelength: 500/570 nm.
    Alizarin Red S sodium
  • HY-D1106
    Py-BODIPY-NHS ester
    		98.98%
    BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells.
    Py-BODIPY-NHS ester
  • HY-136865
    MeOSuc-AAPV-AMC
    		98.07%
    MeOSuc-AAPV-AMC is a fluorogenic substrate for human leukocyte and porcine pancreatic elastase (Km: 362 μM, Ex=380 nm, Em=460 nm).
    MeOSuc-AAPV-AMC
  • HY-D1117
    NADA-green
    NADA-green is a fluorescent D-amino acid probe. NADA-green is efficiently incorporated into the peptidoglycan of diverse bacterial species peptidoglycan biosynthesis. NADA-green allows probing of bacterial growth with minimal perturbation.
    NADA-green
  • HY-D0893
    NSP-SA-NHS
    		99.72%
    NSP-SA-NHS is an acridinium ester that can be used for chemiluminescent immunoassay. A rapid and sensitive chemiluminescent immunoassay of Bisphenol A (BPA) with NSP-SA-NHS-labeled has been developed.
    NSP-SA-NHS
  • HY-D1903
    Fura Red AM
    Fura Red AM is a fluorescent probe that can be used to visualize Ca2+ distribution in the cytoplasm of undifferentiated cells. Fura Red AM operates at an excitation wavelength of 488 nm.
    Fura Red AM
  • HY-137784
    Boc-Val-Pro-Arg-AMC hydrochloride
    		99.43%
    Boc-Val-Pro-Arg-AMC hydrochloride is a sensitive fluorogenic substrate for measuring trypsin-like serine proteases activity.
    Boc-Val-Pro-Arg-AMC hydrochloride
  • HY-W040230
    Acrylodan
    Acrylodan, reacted with thiols, is sensitive to the local environmental dipolarity and dynamics within the binding pocket surrounding Cys34.
    Acrylodan
  • HY-131498
    MOCAc-PLGL(Dpa)AR
    		99.86%
    MOCAc-PLGL(Dpa)AR is a positively charged fluorescent substrate for matrix metalloproteinase-2 (MMP-2), MMP-7 and MMP-9. MOCAc-PLGL(Dpa)AR is a substrate of matrilysin, can be cleaved at the peptide bond between the glycine and leucine residues.
    MOCAc-PLGL(Dpa)AR
Cat. No. Product Name / Synonyms Application Reactivity