2. Signaling Pathways
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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-W040143
    2',7'-Dichlorofluorescein
    		99.22%
    2',7'-Dichlorofluorescein is an important fluorescent product in the detection of oxidative stress and can be used to measure reactive oxygen species (ROS) (Ex=503 nm and Em=523 nm).
    2',7'-Dichlorofluorescein
  • HY-D0047
    5-CFDA
    		98.22%
    5-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase. CF containing portion contains an additional negative charge so that it is better retained in cells, compared to fluorescein dyes.
    5-CFDA
  • HY-19204
    Zinc phthalocyanine
    		≥98.0%
    Zinc phthalocyanine (ZnPc) is commonly applied in industry (catalysts, photoconductors) and biomedical (photodynamic therapy, PDT). Zinc phthalocyanine can be used to photooxidise cyclohexane and is promising for research of solar-cell applications.
    Zinc phthalocyanine
  • HY-139579
    Pafolacianine
    		99.04%
    Pafolacianine (OTL 38) is a fluorescent imaging agent made of near-infrared dye used in detecting tumor lesions during surgical procedures. Pafolacianine excites at 774 to 775 nm and emits at 794 to 796 nm.
    Pafolacianine
  • HY-123533
    Resorufin
    		≥99.0%
    Resorufin (NSC 12097) is a highly fluorescent pink dye. Resorufin shows favorable properties such as water solubility, red-shifted absorption/emission signals. Resorufin is used for the detection of ROS/RNS and a second analyte.
    Resorufin
  • HY-111377
    Amine-PEG3-Biotin
    		99.79%
    Amine-PEG3-Biotin is a signal amplification label containing a biotin group and a terminal primary amine group.
    Amine-PEG3-Biotin
  • HY-123645
    Rhodamine B hydrazide
    		99.87%
    Rhodamine B hydrazide is a good probe for sulfite, with colorless and non-fluorescent properties. While the emission is related to the concentration of sulfite (5-800 ng/mL; detection limit=1.4 ng/mL (3σ)). Sulfite reduces dissolved oxygen to yield superoxide radicals, which binds to Rhodamine B hydrazide to form Rhodamine B. Rhodamine B hydrazide gives Rhodamine B-like fluorescence in the presence of sulfite, which is enhanced by Tween 80 surfactant micelles. Rhodamine B hydrazide has an absorption maximum at 554 nm and a fluorescence emission maximum at 574 nm.
    Rhodamine B hydrazide
  • HY-D0073
    2,3-Diaminonaphthalene
    		99.44%
    2,3 Diaminonaphthalene is a highly selective colorimetric and fluorometric reagent for selenium ion detection and also used for the fluorometric determination of nitrite.
    2,3-Diaminonaphthalene
  • HY-D1807
    Vari Fluor 555-Streptavidin
    Vari Fluor 555-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=555 nm/565 nm.
    Vari Fluor 555-Streptavidin
  • HY-D1696
    MitoTracker Orange CMTMRos
    MitoTracker Orange CMTMRos is a fluorescent dye that labels mitochondria within live cells utilizing the mitochondrial membrane potential (Ex/Em: 551/576 nm).
    MitoTracker Orange CMTMRos
  • HY-D0944
    Giemsa stain
    Giemsa stain can stain chromatin and nuclear membrane. Giemsa stain histopathologic detection of malaria and other microorganisms, such as Histoplasma, LeishmaniaToxoplasma, and Pneumocystis.
    Giemsa stain
  • HY-W011009
    PTCDA
    		≥98.0%
    PTCDA is an organic dye molecule and an organic semiconductor.
    PTCDA
  • HY-D1458
    Peroxyfluor 1
    Peroxyfluor 1 is a cell-permeable probe for H2O2. Peroxyfluor 1 represents a first-generation, green-fluorescent probe.
    Peroxyfluor 1
  • HY-D1819
    Vari Fluor 633-Phalloidin
    Vari Fluor 633-Phalloidin is a fluorescent derivative of Phalloidin that specifically labels myofilament proteins and exhibits red fluorescence at 630/650 nm when labeled.
    Vari Fluor 633-Phalloidin
  • HY-D1159
    HKOH-1r
    		98.43%
    HKOH-1r is a highly sensitive green fluorescent probe for the specific detection of ·OH in living cells with a maximum excitation wavelength and emission wavelength of 500 nm and 520 nm, respectively.
    HKOH-1r
  • HY-118135
    4-Methylumbelliferyl-α-D-galactopyranoside
    		99.76%
    4-Methylumbelliferyl-α-D-galactopyranoside (4MU-α-Gal), a substrate for α-galactosidase A (GLA), is a blue pro-fluorogenic substrate. 4-Methylumbelliferyl-α-D-galactopyranoside forms two products, galactose and fluorescent 4MU, upon cleavage by GLA.
    4-Methylumbelliferyl-α-D-galactopyranoside
  • HY-D1711
    MHI-148
    		98.39%
    MHI-148 is a near-infrared heptamethine cyanine dye with tumor-targeting properties for cancer detection, diagnosis and research. MHI-148 is immediately taken up and accumulated by lysosomes and mitochondria of tumor cells, but not in lysosomes and mitochondria of normal cells.
    MHI-148
  • HY-123629
    TAMRA-PEG3-Azide
    TAMRA-PEG3-Azide is a dye derivative of TAMRA (HY-135640) containing 3 PEG units. TAMRA-PEG3-Azide contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    TAMRA-PEG3-Azide
  • HY-D1428
    Di-4-ANEPPDHQ
    		99.00%
    Di-4-ANEPPDHQ, a fluorescent probe for the visualisation of membrane microdomains in living Arabidopsis thaliana cells.
    Di-4-ANEPPDHQ
  • HY-D1563
    CypHer 5
    CypHer 5 is a pH-sensitive cyanine derivative and a environmental sensitive fluorophore. CypHer 5 has a pH-sensitive maximal absorption at 644 nm and emission at 664 nm. CypHer 5 has been used as a pH sensor of lysosomal compartments.
    CypHer 5
Cat. No. Product Name / Synonyms Application Reactivity