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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-111377
    Amine-PEG3-Biotin
    		99.79%
    Amine-PEG3-Biotin is a signal amplification label containing a biotin group and a terminal primary amine group.
    Amine-PEG3-Biotin
  • HY-D1781
    NTPAN-MI
    		98.78%
    NTPAN-MI is a fluorescent probe. NTPAN-MI is selectively activated upon labeling unfolded proteins with exposed thiols, thereby reporting on the extent of proteostasis.
    NTPAN-MI
  • HY-D1458
    Peroxyfluor 1
    Peroxyfluor 1 is a cell-permeable probe for H2O2. Peroxyfluor 1 represents a first-generation, green-fluorescent probe.
    Peroxyfluor 1
  • HY-W040143
    2',7'-Dichlorofluorescein
    		99.22%
    2',7'-Dichlorofluorescein is an important fluorescent product in the detection of oxidative stress and can be used to measure reactive oxygen species (ROS) (Ex=503 nm and Em=523 nm).
    2',7'-Dichlorofluorescein
  • HY-D1806
    Vari Fluor 594-Streptavidin
    Vari Fluor 594-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=590 nm/617 nm.
    Vari Fluor 594-Streptavidin
  • HY-D0006
    Bathophenanthroline
    		99.76%
    Bathophenanthroline is a nitrogen-containing organic ligand that can form stable complexes with transition metal ions such as Ru2+. In proteomics, Bathophenanthroline is often used for in-gel protein staining. The negatively charged sulfonic acid groups of Bathophenanthroline interact electrostatically with the positively charged amino acid residues of proteins, thereby achieving specific binding to proteins and enhancing the fluorescent signal of detection. Bathophenanthroline can be used for protein separation and detection in SDS-PAGE gels in the field of proteomics, as well as other fluorescence studies[1]. Ex/Em=532 nm/610 nm[1].
    Bathophenanthroline
  • HY-139579
    Pafolacianine
    		99.04%
    Pafolacianine (OTL 38) is a fluorescent imaging agent made of near-infrared dye used in detecting tumor lesions during surgical procedures. Pafolacianine excites at 774 to 775 nm and emits at 794 to 796 nm.
    Pafolacianine
  • HY-D1428
    Di-4-ANEPPDHQ
    		99.00%
    Di-4-ANEPPDHQ is a polarity-sensitive membrane probe with a doubly positive charge that can be used to visualize membrane microdomains in living Arabidopsis cells. Di-4-ANEPPDHQ produces a fluorescence spectrum shift by changing the polarity of the lipid environment after embedding into the cell membrane, thereby quantifying the degree of lipid accumulation. The excitation wavelength of Di-4-ANEPPDHQ is 488 nm, and the emission wavelengths are 560 nm and 650 nm in the liquid ordered and disordered phases, respectively. Di-4-ANEPPDHQ is often used for fluorescence imaging studies of membrane microstructure, lipid order, and membrane tension response in living cells[1][2][3].
    Di-4-ANEPPDHQ
  • HY-118155
    9-Amino-6-chloro-2-methoxyacridine
    		99.56%
    9-Amino-6-chloro-2-methoxyacridine is a pH sensitive fluorescent probe. 9-Amino-6-chloro-2-methoxyacridine has been frequently used to measure changes in vacuolar pH when a specific substrate crosses the tonoplast through a putative H+/solute antiport system.
    9-Amino-6-chloro-2-methoxyacridine
  • HY-W247131
    DASPEI
    		99.86%
    DASPEI is a cationic styrenyl mitochondrial dye with large Stokes shift. DASPEI has excitation and emission wavelength at 550/573 nm, which has good light chromogenic property. DASPEI can stain mitochondria in living cells with good labeling property. And DASPEI can also be used to stain presynaptic nerve endings independently of neuronal activity.
    DASPEI
  • HY-D0073
    2,3-Diaminonaphthalene
    		99.44%
    2,3 Diaminonaphthalene is a highly selective colorimetric and fluorometric reagent for selenium ion detection and also used for the fluorometric determination of nitrite.
    2,3-Diaminonaphthalene
  • HY-D1819
    Vari Fluor 633-Phalloidin
    Vari Fluor 633-Phalloidin is a fluorescent derivative of Phalloidin that specifically labels myofilament proteins and exhibits red fluorescence at 630/650 nm when labeled.
    Vari Fluor 633-Phalloidin
  • HY-15558A
    Hoechst 33258 trihydrochloride
    		99.51%
    Hoechst 33258 trihydrochloride is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
    Hoechst 33258 trihydrochloride
  • HY-15941
    5(6)-FITC
    5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of a fluorescent dye, characterized by high absorbance and excellent fluorescence quantum yield. The isothiocyanate group of FITC can react with various functional groups on proteins, including amines, thiols, imidazoles, tyrosines and carbonyls, enabling the labeling of proteins such as antibodies and lectins. 5(6)-FITC has a wide range of applications, including flow cytometry, immunofluorescence, protease assays and conjugation. The maximum excitation/emission wavelengths are 492/518 nm.
    5(6)-FITC
  • HY-101888
    Cresyl Violet acetate
    Cresyl Violet acetate is a dye, which can be used to stain neurons.
    Cresyl Violet acetate
  • HY-123629
    TAMRA-PEG3-Azide
    TAMRA-PEG3-Azide is a dye derivative of TAMRA (HY-135640) containing 3 PEG units. TAMRA-PEG3-Azide contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    TAMRA-PEG3-Azide
  • HY-W011009
    PTCDA
    		≥98.0%
    PTCDA is an organic dye molecule and an organic semiconductor.
    PTCDA
  • HY-D1742
    DRAQ5
    		Chemical
    DRAQ5 is a novel cell permeant and far red-fluorescing DNA probe. DRAQ5 excites at a wavelength of 647 nm, close to the Ex, and produces a fluorescence spectrum extending from 665 nm out to beyond 780 nm wavelengths. DRAQ5 fluorescence reflects cellular DNA content. DRAQ5 can be used in combination with FITC and RPE-labelled antibodies, without the need for fluorescence compensation.
    DRAQ5
  • HY-131498
    MOCAc-PLGL(Dpa)AR
    		99.86%
    MOCAc-PLGL(Dpa)AR is a positively charged fluorescent substrate for matrix metalloproteinase-2 (MMP-2), MMP-7 and MMP-9. MOCAc-PLGL(Dpa)AR is a substrate of matrilysin, can be cleaved at the peptide bond between the glycine and leucine residues.
    MOCAc-PLGL(Dpa)AR
  • HY-130735
    Janelia Fluor® 646, SE
    		99.67%
    Janelia Fluor 646, SE (JF646, SE) is a red fluorescent dye can be used in cellular imaging. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.
    Janelia Fluor® 646, SE
Cat. No. Product Name / Synonyms Application Reactivity