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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D1696
    MitoTracker Orange CMTMRos
    MitoTracker Orange CMTMRos is a fluorescent dye that labels mitochondria within live cells utilizing the mitochondrial membrane potential (Ex/Em: 551/576 nm).
    MitoTracker Orange CMTMRos
  • HY-D0048
    5-TAMRA-SE
    		98.25%
    5-TAMRA-SE is an amine-reactive fluorescent agent, and its conjugate produces bright, pH-insensitive orange-red fluorescence with good photostability (Ex/Em = 565/580 nm).
    5-TAMRA-SE
  • HY-D0722
    5(6)-CFDA
    		99.31%
    5(6)-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase. CF containing portion contains an additional negative charge so that it is better retained in cells, compared to fluorescein dyes.
    5(6)-CFDA
  • HY-19204
    Zinc phthalocyanine
    		≥98.0%
    Zinc phthalocyanine (ZnPc) is commonly applied in industry (catalysts, photoconductors) and biomedical (photodynamic therapy, PDT). Zinc phthalocyanine can be used to photooxidise cyclohexane and is promising for research of solar-cell applications.
    Zinc phthalocyanine
  • HY-D0367
    Fluorescent Brightener 28 (25% in water)
    Fluorescent brightener 28 is a fluorescent whitening agent commonly used in the padding process of the textile industry. Fluorescent brightener 28 is capable of staining polysaccharides such as cellulose, and when the plasma membrane ruptures, it also weakly stains the cytoplasm and strongly stains the cell nucleus. Additionally, Fluorescent brightener 28 can be utilized to detect intracellular chitin in living cells. Fluorescent Brightener 28 also is a visible light emitting diode (LED)-light sensitive photoinitiator for free radical photopolymerizations.
    Fluorescent Brightener 28 (25% in water)
  • HY-123645
    Rhodamine B hydrazide
    		99.87%
    Rhodamine B hydrazide is a fluorescent derivative based on rhodamine B, containing the spirocyclic structure of Rhodamine B (HY-Y0016), which can be used to detect copper ions (Cu2+), mercury ions, peroxynitrite, hydroxyl radicals and nitric oxide (NO).
    Excitation/emission wavelength:
    Conventional detection: 510/578 nm.
    Sulfite detection: 554 nm absorption, 574 nm emission (due to the formation of Rhodamine B fluorescent product).
    Rhodamine B hydrazide
  • HY-P1005
    Ac-DEVD-AFC
    		99.59%
    Ac-DEVD-AFC is a fluorogenic substrate (λex=400 nm, λem=530 nm).
    Ac-DEVD-AFC
  • HY-D1817
    Vari Fluor 488-Phalloidin
    Vari Fluor 488-Phalloidin is a fluorescent derivative of Phalloidin that specifically labels myofilament proteins and exhibits green fluorescence when labeled, allowing for fluorescence imaging using FITC channels (Ex/Em=488 nm/513 nm).
    Vari Fluor 488-Phalloidin
  • HY-140947
    TAMRA-Azide-PEG-biotin
    		≥99.0%
    TAMRA-Azide-PEG-biotin is a dye derivative of TAMRA (HY-135640). TAMRA-Azide-PEG-biotin contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    TAMRA-Azide-PEG-biotin
  • HY-D1005H
    Poloxamer 188 (F68)
    Poloxamer 188 is a nonionic linear copolymer with surfactant properties. Poloxamer 188 exhibits anti-thrombotic, anti-inflammatory, and cytoprotective activities in various tissue injury models.
    Poloxamer 188 (F68)
  • HY-D1450
    IR-1048
    IR-1048 is a nitroreductase (NTR)-responsive near-infrared fluorescence (NIR)/photoacoustic (PA) imaging probe and photothermal agent. IR-1048 is coupled with a nitroimidazole group to form IR-1048-MZ. IR-1048-MZ is catalytically reduced by NTR in a hypoxic environment, restoring strong near-infrared absorption and fluorescence emission (NIR II window), while activating the photothermal effect. IR-1048 relies on NTR-mediated electron transfer to relieve intramolecular fluorescence quenching, achieving specific imaging and photothermal ablation of tumor hypoxic areas. IR-1048 is mainly used for high-contrast NIR II/photoacoustic imaging and photothermal therapy research and tumor diagnosis of the tumor hypoxic microenvironment.
    IR-1048
  • HY-B1571
    Bromophenol blue
    Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. Bromophenol blue is used as a sensor for the determination of several compounds including ammonia, drugs, proteins and amino acids. The binding of Bromophenol blue to proteins is accompanied by the appearance of a strong absorbance at 610 nm.
    Bromophenol blue
  • HY-D1119C
    AF647-NHS ester triTEA
    		99.74%
    AF647-NHS ester is an analogue of Alexa Fluor 647 (AF647), which has excitation wavelength (λex) of 635 nm (conventional fluorescence detection)/620 nm (instantaneous detection).
    AF647-NHS ester triTEA
  • HY-W127715
    Lucifer Yellow CH dipotassium salt
    		99.87%
    Lucifer Yellow CH dipotassium is a high-intensity fluorescent probe containing free hydrazyl groups. Lucifer Yellow CH can react with fatty aldehydes at room temperature. Lucifer Yellow CH serves as a biological tracer to monitor neuronal branching, regeneration, gap junction detection and characterization, and selective ablation of cells after aldehyde fixation. Lucifer yellow CH displays the maximum excitation/emission of 430 nm/540 nm, respectively.
    Lucifer Yellow CH dipotassium salt
  • HY-118135
    4-Methylumbelliferyl-α-D-galactopyranoside
    		99.76%
    4-Methylumbelliferyl-α-D-galactopyranoside (4MU-α-Gal), a substrate for α-galactosidase A (GLA), is a blue pro-fluorogenic substrate. 4-Methylumbelliferyl-α-D-galactopyranoside forms two products, galactose and fluorescent 4MU, upon cleavage by GLA.
    4-Methylumbelliferyl-α-D-galactopyranoside
  • HY-123533
    Resorufin
    		≥99.0%
    Resorufin (NSC 12097) is a highly fluorescent pink dye. Resorufin shows favorable properties such as water solubility, red-shifted absorption/emission signals. Resorufin is used for the detection of ROS/RNS and a second analyte.
    Resorufin
  • HY-W010042
    L-Glucose
    		99.88%
    L-Glucose (L-(-)-Glucose) is a stereoisomer of D-Glucose (HY-B0389), which does not readily enter the brain. L-Glucose can promote food intake. L-glucose is combined with a fluorescence detector to produce a fluorescent probe that can be used to visualize and characterize cancer cells. L-Glucose also can be used in the research to enhance memory in mice.
    L-Glucose
  • HY-D1506
    Fl-DIBO
    		99.28%
    Fl-DIBO (fluorogenic dibenzocyclooctyne) is a selective and high sensitivity fluorescent probe to azide compounds. Fl-DIBO can react rapidly with azide compounds to form new highly fluorescent products with a maximum emission wavelength of 469 nm and excitation wavelength of 363 nm. Fl-DIBO can be used to label diazo-tagged proteins without detectable background signal interference. Fl-DIBO is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
    Fl-DIBO
  • HY-D1807
    Vari Fluor 555-Streptavidin
    Vari Fluor 555-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=555 nm/565 nm.
    Vari Fluor 555-Streptavidin
  • HY-D0084
    3,3'-Dihexyloxacarbocyanine iodide
    		99.62%
    3,3'-Dihexyloxacarbocyanine iodide is a carbocyanine dye which can be used to monitor changes in mitochondrial membrane potential.
    3,3'-Dihexyloxacarbocyanine iodide
Cat. No. Product Name / Synonyms Application Reactivity