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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-137276
    5-Bromo-3-indolyl β-D-galactopyranoside
    		99.73%
    5-Bromo-3-indolyl β-D-galactopyranoside (Bluo-Gal) is a chromogenic substrate for β-galactosidase. 5-Bromo-3-indolyl β-D-galactopyranoside is hydrolyzed by the enzyme to generate a 5-bromoindole intermediate, which is further oxidized to form an insoluble blue precipitate. 5-Bromo-3-indolyl β-D-galactopyranoside can specifically recognize bacterial β-galactosidases (such as the product of the Escherichia coli lacZ gene) and reacts at pH 7.4, making it suitable for light and electron microscopic observations. 5-Bromo-3-indolyl β-D-galactopyranoside can be used in histochemical detection of reporter gene expression in transgenic organisms, such as the localization analysis of β-galactosidase activity in mouse embryos or muscle tissues.
    5-Bromo-3-indolyl β-D-galactopyranoside
  • HY-137834
    Gly-Pro-AMC hydrobromide
    		98.68%
    Gly-Pro-AMC hydrobromide is a fluorescent dye, it can be used as a specific fluorescent substrate for detecting Dipeptidyl peptidase IV (DPP-IV) activity.
    Gly-Pro-AMC hydrobromide
  • HY-110210
    BODIPY-FL NHS ester
    		99.80%
    BODIPY FL NHS ester (BODIPY FL, SE) is a cell membranes-penatrable amine-reactive fluorescent probe. The maximum excitation/emission wavelength of the BODIPY-FL NHS ester are 502/511 nm, respectively. BODIPY-FL NHS ester has high stability and is insensitive to the polarity, pH and type of solvent, and can maintain stable fluorescence properties under different environmental conditions. BODIPY-FL NHS ester can be used for the synthesis of protease substrates, live cell imaging, protein labeling and immunoassay.
    BODIPY-FL NHS ester
  • HY-D0048
    5-TAMRA-SE
    		98.25%
    5-TAMRA-SE is an amine-reactive fluorescent agent, and its conjugate produces bright, pH-insensitive orange-red fluorescence with good photostability (Ex/Em = 565/580 nm).
    5-TAMRA-SE
  • HY-P0028
    Phalloidin
    		99.83%
    Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome .
    Phalloidin
  • HY-101882
    BCECF
    BCECF is a pH-sensitive fluorescent dye. BCECF allows measurements in the physiological pH range 6.0–8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm.
    BCECF
  • HY-D1617
    BODIPY 500/510 C1, C12
    		99.16%
    BODIPY 500/510 C1, C12 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells. Maximum excitation/emission wavelength: 500/510 nm. Protect from light, stored at -20℃.
    BODIPY 500/510 C1, C12
  • HY-N1389
    Carmine
    Carmine (Carmine red), a natural red dye extracted from the dried females of the insect Dactylopius coccus var. Costa (cochineal). Carmine is a widely used food additive. Carmine provokes both an immediate hypersensitivity and a delayed systemic response with cutaneous expression.
    Carmine
  • HY-D0995
    7-Methyl-6-thioguanosine
    		99.0%
    7-Methyl-6-thioguanosine (MESG) is a chromogenic substrate that can be converted to 7-methyl-6-thioguanine in the presence of purine nucleoside phosphorylase and inorganic phosphate. 7-Methyl-6-thioguanosine can be used to quantify inorganic phosphate. 7-Methyl-6-thioguanosine can also be used to determine the activities of purine nucleoside phosphorylase and protein phosphatases.
    7-Methyl-6-thioguanosine
  • HY-D1819
    Vari Fluor 633-Phalloidin
    Vari Fluor 633-Phalloidin is a fluorescent derivative of Phalloidin that specifically labels myofilament proteins and exhibits red fluorescence at 630/650 nm when labeled.
    Vari Fluor 633-Phalloidin
  • HY-W127703
    Octadecyl Rhodamine B chloride
    		99.58%
    Octadecyl Rhodamine B chloride is an amphiphilic fluorescent probe with both membrane-binding ability and fluorescent properties, which easily forms aggregates in aqueous phase leading to self-quenching. Octadecyl Rhodamine B chloride can be used for cell membrane staining and further applied in membrane biology research. The maximum absorption/emission wavelengths of Octadecyl Rhodamine B chloride monomer in Triton X-100 are 565 nm/585 nm; the absorption peaks of aggregates in water are blue-shifted to 524 nm and 574 nm, with significantly quenched fluorescence.
    Octadecyl Rhodamine B chloride
  • HY-P1005
    Ac-DEVD-AFC
    		99.59%
    Ac-DEVD-AFC is a fluorogenic substrate (λex=400 nm, λem=530 nm).
    Ac-DEVD-AFC
  • HY-101883
    BCECF-AM
    		≥99.0%
    BCECF-AM is a cell membrane permeable compound widely used as a fluorescent indicator for intracellular pH. BCECF-AM could diffuse through the cell membrane and intracellular esterase cleave the ester bond releasing BCECF (HY-101882). BCECF allows measurements in the physiological pH range 6.0-8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm.
    BCECF-AM
  • HY-B0171
    Antipyrine
    		99.98%
    Antipyrine (Phenazone) is an orally active antipyretic and analgesic. Antipyrine can be used as a probe agent for oxidative agent metabolism. Antipyrine also delays gastric emptying (GE) in rats.
    Antipyrine
  • HY-15942
    5-TAMRA
    		98.51%
    5-TAMRA can produce bright, pH-insensitive orange-red fluorescence (excitation and emission extremes of 546/579) and has good photostability. 5-TAMRA is mainly used as a fluorescent marker for the synthesis and study of specific oligonucleotide probes.
    5-TAMRA
  • HY-P0019A
    Z-Gly-Gly-Arg-AMC acetate
    		99.84%
    Z-Gly-Gly-Arg-AMC acetate is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP) (Ex/Em = 390/480 nm).
    Z-Gly-Gly-Arg-AMC acetate
  • HY-126823
    Phen Green SK diacetate (5/6-mixture)
    Phen green SK (PGSK) diacetate (PGSK diacetate (5/6-mixture)) is a metal ion-sensitive fluorescent probe that can penetrate cell membranes. Phen green SK (PGSK) diacetate can react with a variety of metal ions, including Fe2+, Cd2+, Co2+, Ni2+, Zn2+, etc. Phen green SK (PGSK) diacetate chelates Fe2+, resulting in fluorescence quenching, which can be restored when a membrane-permeable chelator is added, thereby reflecting the changes in the intracellular chelatable iron pool. The excitation/emission maxima of Phen green SK diacetate are 507/532 nm, respectively.
    Phen Green SK diacetate (5/6-mixture)
  • HY-D1040
    Cy5.5-COOH
    		98.99%
    Cy5.5-COOH (Cyanine 5.5 carboxylic acid) is a fluorescent dye that emits fluorescence at 710 nm upon excitation at 650 nm. Cy5.5-COOH possesses excellent spectral properties, including a narrow absorption spectrum, high sensitivity, and stability. Cy5.5-COOH can be used for bioimaging and disease diagnosis.
    Cy5.5-COOH
  • HY-121608
    Lumiflavin
    		99.79%
    Lumiflavin (Lumiflavine), a riboflavine analog, causes significant inhibition of riboflavine uptake. Lumiflavin can effectively reduce the riboflavin enrichment in cancer stem-like cells (CSCs) and sensitize the effect of cisplatin Diamminedichloroplatinum (DDP) on CSCs. Lumiflavin is promising for research of ovarian cancer.
    Lumiflavin
  • HY-D1249
    Calcein (mixture of isomers)
    Calcein mixture of isomers is a calcium-dependent fluorescent molecule. Calcein mixture of isomers can be used to study bone metabolism (in vivo) and to stain depressed areas (in vitro). Calcein mixture of isomers can also be used for fluorometry and EDTA titration of calcium ion.
    Calcein (mixture of isomers)
Cat. No. Product Name / Synonyms Application Reactivity