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  4. 5-TAMRA

5-TAMRA can produce bright, pH-insensitive orange-red fluorescence (excitation and emission extremes of 546/579) and has good photostability. 5-TAMRA is mainly used as a fluorescent marker for the synthesis and study of specific oligonucleotide probes.

For research use only. We do not sell to patients.

5-TAMRA

5-TAMRA Chemical Structure

CAS No. : 91809-66-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
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Based on 2 publication(s) in Google Scholar

5-TAMRA Related Antibodies

Top Publications Citing Use of Products

2 Publications Citing Use of MCE 5-TAMRA

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

5-TAMRA can produce bright, pH-insensitive orange-red fluorescence (excitation and emission extremes of 546/579) and has good photostability. 5-TAMRA is mainly used as a fluorescent marker for the synthesis and study of specific oligonucleotide probes[1].

In Vitro

5-TAMRA is converted to hydroxyproline alcohol phosphoramidite reagents, that are suitable for oligonucleotide synthesis. These reagents can be used for automatical synthesis of oligonucleotide probes for DNA analysis. The fluorescence quantum yield of 5-TAMRA is related to the presence of adjacent guanine nucleotides in the oligonucleotide sequence[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

5-TAMRA Related Antibodies
Molecular Weight

430.45

Formula

C25H22N2O5
CAS No.
Appearance

Solid

Color

Green to dark green

Emission (Em)

580
Excitation (Ex)

565
SMILES

O=C(C1=C(C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4)C=CC(C(O)=O)=C1)[O-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (116.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3232 mL 11.6158 mL 23.2315 mL
5 mM 0.4646 mL 2.3232 mL 4.6463 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3232 mL 11.6158 mL 23.2315 mL 58.0788 mL
5 mM 0.4646 mL 2.3232 mL 4.6463 mL 11.6158 mL
10 mM 0.2323 mL 1.1616 mL 2.3232 mL 5.8079 mL
15 mM 0.1549 mL 0.7744 mL 1.5488 mL 3.8719 mL
20 mM 0.1162 mL 0.5808 mL 1.1616 mL 2.9039 mL
25 mM 0.0929 mL 0.4646 mL 0.9293 mL 2.3232 mL
30 mM 0.0774 mL 0.3872 mL 0.7744 mL 1.9360 mL
40 mM 0.0581 mL 0.2904 mL 0.5808 mL 1.4520 mL
50 mM 0.0465 mL 0.2323 mL 0.4646 mL 1.1616 mL
60 mM 0.0387 mL 0.1936 mL 0.3872 mL 0.9680 mL
80 mM 0.0290 mL 0.1452 mL 0.2904 mL 0.7260 mL
100 mM 0.0232 mL 0.1162 mL 0.2323 mL 0.5808 mL
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5-TAMRA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-TAMRA91809-66-4Fluorescent DyeInhibitorinhibitorinhibit

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