2. Apoptosis Autophagy Metabolic Enzyme/Protease Immunology/Inflammation NF-κB Cytoskeleton
  3. Apoptosis Autophagy Reactive Oxygen Species Annexin A NF-κB
  4. Linsidomine hydrochloride

Linsidomine hydrochloride  (Synonyms: SIN-1 chloride)

Cat. No.: HY-101200 Purity: 99.97%
SDS
COA
Handling Instructions Technical Support

Linsidomine hydrochloride (SIN-1 chloride) is a spontaneous ROS/RNS generator and peroxynitrite donor. Linsidomine hydrochloride is a vasodilator and platelet aggregation inhibitor. Linsidomine hydrochloride induces oxidative stress-induced chondrocyte apoptosis and necrosis. Linsidomine hydrochloride inhibits the migration, proliferation and neointima formation of vascular smooth muscle cells by inhibiting the expression of annexin A2. In addition, low doses of Linsidomine hydrochloride shows protective effects on Zn2+ treated nerve cells.

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Linsidomine hydrochloride Chemical Structure

Linsidomine hydrochloride Chemical Structure

CAS No. : 16142-27-1

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 1 publication(s) in Google Scholar

Other Forms of Linsidomine hydrochloride:

Linsidomine hydrochloride Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Linsidomine hydrochloride (SIN-1 chloride) is a spontaneous ROS/RNS generator and peroxynitrite donor. Linsidomine hydrochloride is a vasodilator and platelet aggregation inhibitor. Linsidomine hydrochloride induces oxidative stress-induced chondrocyte apoptosis and necrosis. Linsidomine hydrochloride inhibits the migration, proliferation and neointima formation of vascular smooth muscle cells by inhibiting the expression of annexin A2. In addition, low doses of Linsidomine hydrochloride shows protective effects on Zn2+ treated nerve cells[1][2][3][4].

In Vitro

Linsidomine hydrochloride (0-5 mM; 0-24 h) reduces the activity of hypertrophic chondrocytes, induces hypertrophic chondrocyte necrosis and apoptosis of non-hypertrophic ATDC5 and C28/I2 cells[1].
Linsidomine hydrochloride (30-1000 μM; 190 min) prevents Zn2+ induced neuronal death, but is eliminated by uric acid (HY-B2130)[2].
Linsidomine hydrochloride (100-300 μM; 0-190 min) increases the production of reactive oxygen species and active nitrogen, inhibits the Zn2+ induced glutathione reductase inactivation and the increase of oxidized glutathione/total glutathione ratio in PC12 cells[2].
Linsidomine hydrochloride (1 mM; 2-3 h) induces nuclear accumulation of Nrf2 in EAhy926 and HUVECs cells[3].
Linsidomine hydrochloride (0-500 μM; 30 min) inhibits platelet-derived growth factor BB-induced cell migration and proliferation by annexin A2 in rat aortic smooth muscle cells[4].
Linsidomine hydrochloride (1-5 mM, 24 h) induces the accumulation of LC3-II, and the formation of autophagosome, inducing autophagy in serum starved EAhy926 cell[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Linsidomine hydrochloride Related Antibodies

Cell Viability Assay[1]

Cell Line: Hypertrophic chondrocytes
Concentration: 0-5 mM
Incubation Time: 24 h
Result: Reduced cellular viability in a dose/time-dependent manner.

Cell Viability Assay[2]

Cell Line: PC12
Concentration: 30 μM-1 mM
Incubation Time: 3 h
Result: Improved Zn2+-reduced cell viability (< 1 mM).
Inhibited cell viability with the dose more than 1 mM.

Western Blot Analysis[4]

Cell Line: serum starved EAhy926
Concentration: 0-5 mM
Incubation Time: 24 h
Result: Improved expression of LC3-II.
In Vivo

Linsidomine hydrochloride (0.01-0.1 mg/kg; ip; 14 days) attenuates neointima formation in balloon-injured rat carotid arteries[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Sprague Dawley rats with a balloon-injured carotid artery model[4]
Dosage: 0.01-0.1 mg/kg
Administration: ip, 14 days
Result: Reduced the I/M (intima to media) ratio of the carotid artery in the group treated with 0.1 mg/kg/day.
Did not change the I/M ratio of the carotid artery in the group treated with 0.01 mg/kg/day.
Molecular Weight

206.63

Formula

C6H11ClN4O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC1=C[N+](N2CCOCC2)=NO1.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (241.97 mM; Need ultrasonic)

DMSO : 25 mg/mL (120.99 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.8395 mL 24.1975 mL 48.3950 mL
5 mM 0.9679 mL 4.8395 mL 9.6790 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.97%

SDS (393 KB)

COA (248 KB) HNMR (256 KB) RP-HPLC (261 KB) LCMS (101 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 4.8395 mL 24.1975 mL 48.3950 mL 120.9875 mL
5 mM 0.9679 mL 4.8395 mL 9.6790 mL 24.1975 mL
10 mM 0.4839 mL 2.4197 mL 4.8395 mL 12.0987 mL
15 mM 0.3226 mL 1.6132 mL 3.2263 mL 8.0658 mL
20 mM 0.2420 mL 1.2099 mL 2.4197 mL 6.0494 mL
25 mM 0.1936 mL 0.9679 mL 1.9358 mL 4.8395 mL
30 mM 0.1613 mL 0.8066 mL 1.6132 mL 4.0329 mL
40 mM 0.1210 mL 0.6049 mL 1.2099 mL 3.0247 mL
50 mM 0.0968 mL 0.4839 mL 0.9679 mL 2.4197 mL
60 mM 0.0807 mL 0.4033 mL 0.8066 mL 2.0165 mL
80 mM 0.0605 mL 0.3025 mL 0.6049 mL 1.5123 mL
100 mM 0.0484 mL 0.2420 mL 0.4839 mL 1.2099 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Linsidomine hydrochloride Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Linsidomine16142-27-1SIN-1SIN1SIN 1ApoptosisAutophagyReactive Oxygen SpeciesAnnexin ANF-κBAnxANuclear factor-κBNuclear factor-kappaBballoon-injured rat carotid arteriesPC12 cellsEAhy926 cellsHUVECs cellsvascular smooth muscle cellsInhibitorinhibitorinhibit

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