2. Signaling Pathways
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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D0454
    C.I. Disperse red 54
    C.I. Disperse red 54 is a red dye commonly used for fiber dyeing.
    C.I. Disperse red 54
  • HY-117445
    Oxazole yellow
    Oxazole yellow is a cyanine dye composed of benzoxazole and quinoline rings connected by a linker. It is almost non-luminescent in water, but its green fluorescence is significantly enhanced after intercalation in double-stranded DNA. Oxazole yellow can be used to detect cell apoptosis.
    Oxazole yellow
  • HY-D2508
    Cy3-PEG-NH2 (MW 10000)
    Cy3-PEG-NH2 (MW 10000) is a polyethylene glycol derivative containing Cy3 (HY-D0822) fluorescent dye and polyethylene glycol (PEG) and an amino group. The Cy3 fluorophore is commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. Cy3 has an emission maximum around 562-570 nm. Cy3-PEG-NH2 (MW 10000) can be used for fluorescence imaging, fluorescence tracing and fluorescence labeling.
    Cy3-PEG-NH2 (MW 10000)
  • HY-D2297
    AIE-GA
    AIE-GA is a Golgi apparatus (GA) fluorescent probe (green channel: λex = 405 nm, λem = 500-700 nm). AIE-GA has a favourable binding ability to interact with COX-2. AIE-GA binds to the cyclooxygenase catalytic site of COX-2.
    AIE-GA
  • HY-D2760
    BP Fluor 488 acid
    BP Fluor 488 acid is a powerful labeling dye. It will react with the amine group in antibody, proteins, peptides, amino-modified oligos, and other target molecules. The dye has an excitation peak at 499 nm and an emission peak at 520 nm. The conjugates are widely used in microscopy, flow cytometry, and other applications.
    BP Fluor 488 acid
  • HY-D0637
    Disperse Blue 106
    Disperse Blue 106 is a yellow dye commonly used for fiber dyeing.
    Disperse Blue 106
  • HY-W415108
    10-Methyl-9-(phenoxycarbonyl) acridinium trifluoromethanesulfonate
    		99.41%
    10-Methyl-9-(phenoxycarbonyl) acridinium trifluoromethanesulfonate is an acridinium ester that produces fluorescent 10-methyl-9-acridone upon oxidation with hydrogen peroxide, persulfate, and other oxidants under alkaline conditions. 10-Methyl-9-(phenoxycarbonyl) acridinium trifluoromethanesulfonate can be used in chemiluminescent assays, enzyme, antigen, antibody, and hormone immunoassays, and for the detection of oxidants in environmental, biological, and pharmaceutical samples.
    10-Methyl-9-(phenoxycarbonyl) acridinium trifluoromethanesulfonate
  • HY-D0431
    C.I. Pigment blue 56
    C.I. Pigment blue 56 is the most important colorant used to add color or change the color of something and is chemically essentially unaffected by the carrier or medium into which it is incorporated. It can be widely used in textile, medicine, food, cosmetics, plastics, paint, ink, photography and paper industries.
    C.I. Pigment blue 56
  • HY-D2656
    Cy5.5 HA (MW 3000)
    Cy5.5 HA (MW 3000) is a hyaluronic acid labeled with CY5.5 (HY-D0924). Cy5.5 HA (MW 3000) is widely used for cell imaging and in vivo imaging of shallow tissue (Ex/Em = 680/710 nm).
    Cy5.5 HA (MW 3000)
  • HY-D0594
    Disperse Red 278
    Disperse Red 278 is a red dye derived from anthraquinone. It is used in plastics, textiles and cosmetics.
    Disperse Red 278
  • HY-D2738
    BP Fluor 532 NHS ester
    BP Fluor 532 NHS ester is an amine reactive, yellow-emitting dye routinely used to label proteins or antibodies through primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond. BP Fluor 532 is a bright yellow-fluorescent dye with pH insensitive emission from pH 4 to pH 10. The excitation of BP Fluor 532 is ideally suited for the frequency-doubled Nd:YAG laser line. BP Fluor 532 can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is often used for the generation of stable signals in imaging and flow cytometry.
    BP Fluor 532 NHS ester
  • HY-D0510
    C.I. Pigment orange 34
    C.I. Pigment orange 34 is the most important colorant used to add color or change the color of something and is chemically essentially unaffected by the carrier or medium into which it is incorporated. It can be widely used in textile, medicine, food, cosmetics, plastics, paint, ink, photography and paper industries.
    C.I. Pigment orange 34
  • HY-D2750
    Sulfo-Cy5 picolyl azide
    Sulfo-Cy5 Picolyl Azide is a fluorophore featuring a sulfonate group and an azide. Azide groups are click chemistry handles which are reactive towards terminal alkynes and strained cyclooctynes such as BCN or DBCO. Cy5 is a cyanine dye with excitation and emission maxima at 651 nm and 670 nm respectively. The sulfonate group on the Cy5 dye increases this compound’s water solubility.
    Sulfo-Cy5 picolyl azide
  • HY-W800691
    BP Fluor 350 picolyl azide
    BP Fluor 350 Picolyl Azide is a blue-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.
    BP Fluor 350 picolyl azide
  • HY-D0198
    Acid yellow 61
    Acid yellow 61 is an acidic yellow dye that can be adsorbed and removed by bentone clay.
    Acid yellow 61
  • HY-D2651
    Cy5 HA MA (MW 50000)
    Cy5 HA MA (MW 50000) is a hyaluronic acid methacrylate labeled with CY5 (HY-D0821). Cy5 HA MA (MW 50000) is widely used in the fields of biolabeling and imaging, drug delivery and tissue engineering and regenerative medicine (Ex/Em = 633/670 nm).
    Cy5 HA MA (MW 50000)
  • HY-D2763
    BP Fluor 532 maleimide
    BP Fluor 532 maleimide is a photostable, bright yellow-fluorescent dye with pH insensitive emission from pH 4 to pH 10. The excitation of BP Fluor 532 is ideally suited for the frequency-doubled Nd:YAG laser line. BP Fluor 532 dye can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is often used for the generation of stable signals in imaging and flow cytometry. Maleimide is the most popular sulfhydryl-reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form a stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.
    BP Fluor 532 maleimide
  • HY-D0443
    C.I. Direct black 22
    C.I. Direct black 22 is a good dyeing agent for cotton fabrics.
    C.I. Direct black 22
  • HY-D0107
    Fluorescein dicaproate
    Fluorescein dicaproate is a colorimetric probe formed by the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.
    Fluorescein dicaproate
  • HY-D0413
    C.I. Acid red 37
    C.I. Acid red 37 is an acid red dye that can be adsorbed and removed by bentone clay.
    C.I. Acid red 37
Cat. No. Product Name / Synonyms Application Reactivity