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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D2553
    Cy5.5-PEG-NH2 (MW 3400)
    Cy5.5-PEG-NH2 (MW 200) is a polyethylene glycol derivative containing Cy5.5 (HY-D0924) fluorescent dye and polyethylene glycol (PEG) and an amino group. The Cy5.5 fluorophore is commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. Cy5.5 has an emission maximum around 710 nm. Cy5.5-PEG-NH2 (MW 2000) can be used for fluorescence imaging, fluorescence tracing and fluorescence labeling.
    Cy5.5-PEG-NH2 (MW 3400)
  • HY-116070R
    Iodoantipyrine (Standard)
    Iodoantipyrine (Standard) is the analytical standard of Iodoantipyrine. This product is intended for research and analytical applications. Iodoantipyrine (4-Iodoantipyrine) is an iodinated Antipyrine (HY-B0171) with potential antiviral activity. Antipyrine (Phenazone) is an antipyretic and analgesic that also serves as a molecular probe of hepatic oxidative metabolites.
    Iodoantipyrine (Standard)
  • HY-D1202
    Disperse Violet 63
    Disperse Violet 63 is a violet dye commonly used for fiber dyeing.
    Disperse Violet 63
  • HY-D2854D
    FITC-PEG-CL (MW 5000)
    FITC-PEG-CL (MW 5000) is a fluorescent dye composed of FITC (HY-66019), PEG and chlorine (-Cl). The chlorine group is an electrophilic active group that can be used for nucleophilic substitution reactions and facilitate subsequent chemical modification (Ex/Em = 488/525 nm).
    FITC-PEG-CL (MW 5000)
  • HY-143285
    AMC-GlcNAc
    AMC-GlcNAc (compound 1) is a potent fluorogenic probe for detection and characterization of β-hexosaminidase enzyme activity. AMC-GlcNAc enables continuous monitoring by turn-on fluorescence and fluorescence signal is constant over a wide pH range (Ex=325 nm, Em=390 nm). AMC-GlcNAc enables ratiometric fluorescence detection with high sensitivity and low background, which can be used to screen for recombinant dispersin B activity in E. coli cell lysate.
    AMC-GlcNAc
  • HY-D2569
    Cy5.5-PEG-Biotin (MW 2000)
    Cy5.5-PEG-Biotin (MW 2000) is a polyethylene glycol derivative containing Cy5.5 (HY-D0924) fluorescent dye and polyethylene glycol (PEG) and a Biotin. Biotin can bind with high affinity to streptavidin or avidin. Cy5.5-PEG-Biotin (MW 2000) plays an important role in the specific capture and detection of biomolecules.
    Cy5.5-PEG-Biotin (MW 2000)
  • HY-15930R
    TMB (Standard)
    TMB (Standard) is the analytical standard of TMB. This product is intended for research and analytical applications. TMB is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922.
    TMB (Standard)
  • HY-D0233R
    Leucocrystal violet (Standard)
    Leucocrystal violet (Standard) is the analytical standard of Leucocrystal violet. This product is intended for research and analytical applications. Leucocrystal violet is a triphenylmethane dye which can be used to detect antimony in environmental and biological samples using spectrophotometric techniques.
    Leucocrystal violet (Standard)
  • HY-160277
    DSPE-PEG-Fluor 594,MW 2000
    DSPE-PEG-Fluor 594,MW 2000 is a PEGylated lipid with a DSPE group and a Fluor 594 fluorophore. DSPE is a phospholipid. Fluor 594 is a red water-soluble dye.
    DSPE-PEG-Fluor 594,MW 2000
  • HY-172269
    BP Fluor 488
    BP Fluor 488 is a bright, green-fluorescent dye widely used for labeling aldehydes or ketones in polysaccharides or glycoproteins. The dye has an excitation peak at 499 nm and an emission peak at 520 nm. The conjugates are widely used in microscopy, flow cytometry, and other applications.
    BP Fluor 488
  • HY-D0437
    C.I. Direct blue 75
    C.I. Direct blue 75 is a good dyeing agent for cotton fabrics.
    C.I. Direct blue 75
  • HY-D2434
    HRP-PEG-Cy3
    HRP-PEG-Cy3 is a HRP and Cy3 (HY-D0822) labeled PEG. The Cy3 fluorophore is commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. Cy3 has an emission maximum around 562-570 nm.
    HRP-PEG-Cy3
  • HY-D0338
    Direct Yellow 26
    Direct Yellow 26 is a good dyeing agent for cotton fabrics.
    Direct Yellow 26
  • HY-D2670
    Cy5.5 HA biotin 5000
    Cy5.5 HA biotin 5000 is a biotinylated hyaluronic acid labeled with CY5.5 (HY-D0924). Cy5.5 HA biotin 5000 is widely used for cell imaging and in vivo imaging of shallow tissues (Ex/Em = 680/710 nm).
    Cy5.5 HA biotin 5000
  • HY-D0727
    Pigment yellow 174
    Pigment yellow 174 is a disazomethine dye with solid-state fluorescence.a biologically active chemical.
    Pigment yellow 174
  • HY-D0820A
    CY5-YNE triethylamine
    CY5-YNE (Sulfo-Cyanine5-alkyne) triethylamine is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides (Ex: 650 nM; Em: 680 nm). CY5-YNE triethylamine is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
    CY5-YNE triethylamine
  • HY-D1337
    Cyanine7 carboxylic acid chloride
    Cyanine7 carboxylic acid chloride belongs to the cyanine dye series and is a common fluorescent marker for biomolecules that can interact with biomolecules. Cyanine dyes may also bind to double-helical DNA through intercalation and exhibit enhanced fluorescence upon binding.
    Cyanine7 carboxylic acid chloride
  • HY-D0105
    Fluorescein diacetate 6-isothiocyanate
    Fluorescein diacetate 6-isothiocyanate is a colorimetric probe formed by the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.
    Fluorescein diacetate 6-isothiocyanate
  • HY-134311
    8-NBD-cGMP
    8-NBD-cGMP is a fluorescent analog of cyclic guanosine monophosphate and a potent, membrane-permeable, fluorescent activator of cGMP-dependent protein kinase isozymes I α and I β. 8-NBD-cGMP is barely fluorescent in aqueous solution but fluoresces strongly in hydrophobic environments such as hydrophobic protein binding sites
    8-NBD-cGMP
  • HY-172285
    Cy3 hydrazide
    Cy3 hydrazide is a cyanine dye linker containing hydrazide, which can be used to label aldehydes and ketones. This reagent allows to label various carbonyl-containing molecules such as antibodies and other glycoproteins after periodate oxidation, proteins after oxidative stress or deamination, or reducing saccarides. Cyanine3 is compatible with a number of fluorescent instruments.
    Cy3 hydrazide
Cat. No. Product Name / Synonyms Application Reactivity