2. Academic Validation
  3. Deamino-hydroxy-phoslactomycin B, a biosynthetic precursor of phoslactomycin, induces myeloid differentiation in HL-60 cells

Deamino-hydroxy-phoslactomycin B, a biosynthetic precursor of phoslactomycin, induces myeloid differentiation in HL-60 cells

  • Biochem Biophys Res Commun. 2009 Jun 12;383(4):406-10. doi: 10.1016/j.bbrc.2009.04.014.
Siro Simizu 1,  Takayuki Teruya,  Toshihiko Nogawa,  Harumi Aono,  Masashi Ueki,  Masakazu Uramoto,  Yuichi Kobayashi,  Hiroyuki Osada
Affiliations

Affiliation

  • 1 Antibiotics Laboratory and Chemical Biology Department, Advanced Science Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
PMID: 19364501 DOI: 10.1016/j.bbrc.2009.04.014
Abstract

During the screening for novel differentiation inducers, we found that a culture broth of Streptomyces sp. HK-803 induced myeloid differentiation of HL-60 cells. The active substance was identified as deamino-hydroxy-phoslactomycin B (HPLM) by mass spectrometry, and synthesized HPLM also induced the differentiation of HL-60 cells. HPLM showed greater inhibition of protein Phosphatase 2A (PP2A) activity than phoslactomycin B (PLMB); however, PLMB and okadaic acid did not induce differentiation. Moreover, treatment with ATRA and 1alpha, 25(OH)2D3 induced retinoic acid receptor-beta and 1alpha, 25(OH)2D3 24-hydroxylase, respectively, whereas HPLM did not, suggesting that HPLM is a novel differentiation inducer.

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