2. Signaling Pathways
  3. Cell Cycle/DNA Damage
  4. DNA/RNA Synthesis

DNA/RNA Synthesis

DNA/RNA Synthesis

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RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-N0157C
    Orotic acid potassium
    		99.51%
    Orotic acid potassium (Vitamin B13 potassium) is a precursor of pyrimidine bases and is involved in the synthesis of DNA and RNA. Orotic acid potassium stimulates the growth of animals, plants and microorganisms, participates in carbohydrate metabolism, and is necessary for the growth and life activities of organisms. Orotic acid potassium is a measurement indicator in routine newborn screening for urea cycle abnormalities. Orotic acid potassium can cause hepatic steatosis and hepatomegaly in rats.
    Orotic acid potassium
  • HY-135282
    DHODH-IN-1
    		Inhibitor 98.65%
    DHODH-IN-1 (compound 18d) is a potent Dihydroorotate Dehydrogenase (DHODH) inhibitor with an IC50 of 25 nM. DHODH-IN-1 is an inhibitor of pyrimidine biosynthesis pathway.
    DHODH-IN-1
  • HY-W013053
    Dibenz[a,h]anthracene
    		Inducer ≥98.0%
    Dibenz[a,h]anthracene (DBA) is a polycyclic aromatic hydrocarbon (PAH) of considerable tumorigenicity. Dibenz[a,h]anthracene results in DNA adduct formation leading to the activation of a DNA damage response. Dibenz[a,h]anthracene induces cell cycle arrest and apoptosis via both Tp53-dependent and Tp53-independent mechanisms.
    Dibenz[a,h]anthracene
  • HY-158719
    3′-O-Azidomethyl-dATP sodium solution (100mM)
    		99.85%
    3′-O-Azidomethyl-dATP sodium solution (100mM) is a labeled modified deoxyoligonucleotide (dNTP) that can release pyrophosphate to produce fluorescence and has special applications in gene synthesis and sequencing.
    3′-O-Azidomethyl-dATP sodium solution (100mM)
  • HY-145975A
    m7Gpppm6AmpG (ammonium) solution (100mM)
    		98.84%
    m7Gpppm6AmpG ammonium solution (100mM) is a trinucleotide mRNA 5’ cap analogs. m7Gpppm6AmpG ammonium can be used for RNA synthesis in vitro.
    m7Gpppm6AmpG (ammonium) solution (100mM)
  • HY-W009354
    Di-tert-butyl diisopropylphosphoramidite
    Di-tert-butyl diisopropylphosphoramidite is a phosphorite monomer that can be used in the synthesis of oligonucleotides.
    Di-tert-butyl diisopropylphosphoramidite
  • HY-145555
    Bersiporocin
    		Inhibitor 98.99%
    Bersiporocin is a prolyl-tRNA synthetase inhibitor. Bersiporocin has an IC50 of ≤100 nM for phosphoribosylpyrophosphate synthetase (PRS). Bersiporocin can be used for the research of antifibrotic.
    Bersiporocin
  • HY-122903A
    (-)-TK216
    		Inhibitor 99.45%
    (-)-TK216 is an enantiomer of TK216 (HY-122903). TK216 is an orally active and potent E26 transformation specific (ETS) inhibitor. (-)-TK216 has anti-cancer activity.
    (-)-TK216
  • HY-112081
    BAY-707
    		Inhibitor 99.64%
    BAY-707 is a substrate-competitive, highly potent and selective inhibitor of MTH1(NUDT1) with an IC50 of 2.3 nM. BAY-707 has a good pharmacokinetic (PK) profile to other MTH1 compounds and is well-tolerated in mice, but shows a clear lack of in vitro or in vivo anticancer efficacy.
    BAY-707
  • HY-12695B
    Guanosine 5'-triphosphate trisodium salt hydrate
    5'-GTP trisodium salt hydrate is an activator of the signal transducing G proteins and also serves as an energy-rich precursor of mononucleotide units in the enzymatic biosynthesis of DNA and RNA.
    Guanosine 5'-triphosphate trisodium salt hydrate
  • HY-W436424
    DAM-IN-1
    		Inhibitor
    DMA-IN-1 is a DNA adenine methyltransferase (DAM) inhibitor, IC50: 48 μM. DMA-IN-1 inhibits Caulobacter growth with an MIC of 35 μM.
    DAM-IN-1
  • HY-N2566
    Euscaphic acid
    		Inhibitor 98.34%
    Euscaphic acid, a DNA polymerase inhibitor, is a triterpene from the root of the R. alceaefolius Poir. Euscaphic inhibits calf DNA polymerase α (pol α) and rat DNA polymerase β (pol β) with IC50 values of 61 and 108 μM. Euscaphic acid induces apoptosis.
    Euscaphic acid
  • HY-134665
    NITD-2
    		Inhibitor 99.36%
    NITD-2, a dengue virus (DENV) polymerase inhibitor, inhibits the DENV RdRp-mediated RNA elongation. NITD-2 penetrates cell membrane poorly.
    NITD-2
  • HY-121687
    HIPP
    		Inhibitor 99.81%
    HIPP (α-(Hydroxyimino)benzenepropanoic acid) is an inhibitor for human C-terminal binding protein (CtBP), with IC50 of 745 nM and a Kd of 1.3 μM.
    HIPP
  • HY-108999A
    Crisnatol
    		Inhibitor 99.51%
    Crisnatol (BWA770U) is an orally active and anticancer agent, and a member of the arylmethylaminopropanediol class of DNA intercalators. Crisnatol shows in vitro cytotoxicity against human breast cancer cells, but not normal human skin fibroblasts.
    Crisnatol
  • HY-110407
    2'-O,4'-C-Methyleneadenosine
    2'-O,4'-C-Methyleneadenosine (LNA-A) is a locked nucleic acid (LNA) and is also an adenosine analog.
    2'-O,4'-C-Methyleneadenosine
  • HY-107790
    5-Methoxyflavone
    		Inhibitor 99.97%
    5-Methoxyflavone, belonged to Flavonoid family, is a DNA polymerase-beta inhibitor and neuroprotective agent against beta-amyloid toxicity. possess central nervous system (CNS) depressant effect mediated through the ionotropic GABAA receptors.
    5-Methoxyflavone
  • HY-106014
    Tezacitabine
    		Inhibitor 99.41%
    Tezacitabine is a cytostatic and cytotoxic antimetabolite and a nucleoside analogue. Tezacitabine irreversibly inhibits the ribonucleotide reductase and interferes with DNA replication and repair. Tezacitabine effectively induces cells apoptotic. Tezacitabine has the potential for leukemias and solid tumors (carcinomas) treatment.
    Tezacitabine
  • HY-W570886
    2'-O-MOE-U
    2'-O-MOE-U is a phosphoramidite, can be used for oligonucleotide synthesis.
    2'-O-MOE-U
  • HY-N1150R
    Thymidine (Standard)
    		Inhibitor
    Thymidine (Standard) is the analytical standard of Thymidine. This product is intended for research and analytical applications. Thymidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication.
    Thymidine (Standard)
Cat. No. Product Name / Synonyms Application Reactivity