HDAC6-IN-66

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HDAC6-IN-66 is a potent and selective histone deacetylase (HDAC) 6 inhibitor with an IC₅₀ of 1.8 nM. HDAC6-IN-66 induces α-tubulin acetylation over histone H3. HDAC6-IN-66 can be used for the research of cancer.

For research use only. We do not sell to patients.

HDAC6-IN-66 Chemical Structure

CAS No. : 3095060-23-1

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HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target^[1]

HDAC6

1.8 nM (IC₅₀)

HDAC3

73.6 nM (IC₅₀)

HDAC1

104.9 nM (IC₅₀)

In Vitro

HDAC6-IN-66 (Compound 5j) selectively inhibits HDAC6 (IC₅₀ = 1.8 nM), with significantly higher IC₅₀ against other HDAC isoforms (HDAC1 : 104.9 nM, HDAC3 : 73.6 nM; HDAC4 : 271.3 nM)^[1].
HDAC6-IN-66 exhibits significant antiproliferative activity against HCT116 cells (GI₅₀ = 3.1 μM), and shows mild inhibitory activity against other cancer cell lines such as MDA-MB-231 (GI₅₀ = 22.3 μM) and HCC827 cells (GI₅₀ = 15.9 μM)^[1].
HDAC6-IN-66 (0.5-10 μM, 24 h) induces acetylation of α-tubulin in HCT116 and MDA-MB-231 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

333.34

Formula

C₁₉H₁₅N₃O₃

CAS No.

3095060-23-1

SMILES

O=C(NO)C1=CC=C(CN2N=C(C3=CC=CO3)C4=C2C=CC=C4)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Ali KH, et al. Design, synthesis, and biological evaluation of indazole-based histone deacetylase 6 inhibitors. Eur J Med Chem. 2025 Oct 5;295:117785. [Content Brief]

HDAC6-IN-66 Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HDAC6-IN-663095060-23-1HDACHistone deacetylaseshistone deacetylaseα-tubulincancerInhibitorinhibitorinhibit

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