2. Signaling Pathways
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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-W140203
    2-(2′-Hydroxyphenyl)benzimidazole
    		99.92%
    2-(2′-Hydroxyphenyl)benzimidazole is some of the most extensively studied excited state intramolecular proton transfer (ESIPT) molecules exhibiting normal and tautomer emissions. 2-(2′-Hydroxyphenyl)benzimidazole has been applied as a fluorescent probe in various systems.
    2-(2′-Hydroxyphenyl)benzimidazole
  • HY-DN0194F
    Cy3-Asiatic acid
    Cy3-Asiatic acid is a fluorescent labeling reagent that combines Cy3 (HY-D0822) and Asiatic acid (HY-N0194). Cy3-Asiatic acid can be used to observe and track Asiatic acid under fluorescence microscopy (Ex/Em = 550/570 nm).
    Cy3-Asiatic acid
  • HY-D0029
    5(6)-Aminofluorescein
    		98.55%
    5(6)-Aminofluorescein (5(6)-AFM) is a a precursor for synthesis of 5(6)-FITC (HY-15941). 5(6)-FITC is an amine-reactive derivative of fluorescein dye.
    5(6)-Aminofluorescein
  • HY-66020
    6-FITC
    		99.29%
    6-FITC (6-Fluorescein Isothiocyanate) is a derivative of fluorescein used in wide-ranging applications including flow cytometry.
    6-FITC
  • HY-W015996
    4-Nitrophenyl-N-acetyl-α-D-galactosaminide
    		99.92%
    4-Nitrophenyl-N-acetyl-α-D-galactosaminide (pNP-GalNAc) can be used as a chromogenic substrate for N-acetyl-D-galactosaminase. 4-Nitrophenyl-N-acetyl-α-D-galactosaminide is cleaved by N-acetyl-D-galactosaminase to generate a yellow solution. The enzyme activity is quantitatively determined by absorbance detection.
    4-Nitrophenyl-N-acetyl-α-D-galactosaminide
  • HY-D2365
    QSY 21 NHS
    QSY 21 NHS, a dark quencher is an efficient energy transfer acceptor of the far red and NIR fluorescent probes. QSY 21 NHS works in the wavelength range of 540-750 nm, and is frequently used in FRET applications. QSY 21 NHS does not emit fluorescence in normal conditions. NHS esters can be used to label the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.
    QSY 21 NHS
  • HY-129594
    DANP
    DANP is a bulge base recognition probe that strongly and specifically binds to the single cytosine and thymine bulges. DANP could stabilize not only a single cytosine but also the thymine bulge in duplex DNA.
    DANP
  • HY-D0491
    C.I. Disperse blue 79
    C.I. Disperse blue 79 is a blue dye commonly used for fiber dyeing.
    C.I. Disperse blue 79
  • HY-D0278
    Pigment red 170
    Pigment Red 170 is a commonly used commercial dye for bulk coloring of plastics and synthetic fibers and for surface coatings such as paints and inks.
    Pigment red 170
  • HY-113238F
    FITC-Lithocholic acid 3-sulfate
    FITC-Lithocholic acid 3-sulfate (FITC-Sulfolithocholic acid) is a FITC-labeled Lithocholic acid 3-sulfate, which is a sulfated biliary metabolite. Lithocholic acid 3-sulfate selectively inhibits Th17 cell differentiation by targeting RORγt. Lithocholic acid 3-sulfate can be used for the research of pathogenesis of cholestatic liver diseases.
    FITC-Lithocholic acid 3-sulfate
  • HY-129594A
    DANP dihydrochloride
    		99.29%
    DANP dihydrochloride is a bulge base recognition probe that strongly and specifically binds to the single cytosine and thymine bulges. DANP dihydrochloride could stabilize not only a single cytosine but also the thymine bulge in duplex DNA.
    DANP dihydrochloride
  • HY-156832F
    5'6-FAM-GGUGAAAGACGAUGGACAATT,UUGUCCAUCGUCUUUCACCTT
    5'6-FAM-GGUGAAAGACGAUGGACAATT,UUGUCCAUCGUCUUUCACCTT is a double-stranded ribonucleoside modified by the fluorescent molecule 5'6-FAM. 5'6-FAM is a mixture consisting of 5-FAM (HY-66022) and 6-FAM (HY-66021). Ex/Em of 5-FAM = 490 nm/520 nm.
    5'6-FAM-GGUGAAAGACGAUGGACAATT,UUGUCCAUCGUCUUUCACCTT
  • HY-15930C
    TMB monosulfate
    		99.99%
    TMB monosulfate is the monosulfate form of TMB (HY-15930). TMB monosulfate is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB monosulfate can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB monosulfate can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922.
    TMB monosulfate
  • HY-D1052
    Cy7-YNE
    Cy7-YNE is a fluorescence labeling agent (Ex=700-770 nm，Em=790 nm). Cyanine dyes are used to label proteins, antibodies, and peptides. Cy7-YNE is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
    Cy7-YNE
  • HY-101937
    L-ANAP
    L-ANAP is a genetically encodable and polarity-sensitive fluorescent unnatural amino acid (Uaa).
    L-ANAP
  • HY-D1542
    Mucicarmine
    Mucicarmine is used in the histological visualization of acid mucopolysaccharides in tissue sections. Mucicarmine can identify mucin (deep rose), which is useful in determining the mucin positive cancer (such as liver carcinoma).
    Mucicarmine
  • HY-D0942
    Acridine Orange zinc chloride salt
    Acridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm).
    Acridine Orange zinc chloride salt
  • HY-D1054
    Cy2 (iodine)
    Cy2 iodine is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance.
    Cy2 (iodine)
  • HY-D1032
    DiZHSeC
    		99.90%
    DiZHSeC is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells.
    DiZHSeC
  • HY-D0954
    Jenner's Stain
    Jenner's Stain is a dye that is used in microscopy for staining blood smears. Jenner's Stain can be used for the chromosome stain by C-banding technique. Jenner's Stain can be used for the stain for routine blood examinations and malarial staining.
    Jenner's Stain
Cat. No. Product Name / Synonyms Application Reactivity